LOCUS       sequence-137536-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN plasmid as receiving vector for exogenous pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pRS414-URR
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             150..161
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(203..209,210..227,228..233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    248..269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    370..391
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt
       61 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg
      121 ataacgtctt cggaggaagc catctagtat ttctcctctt tctctagtat gtgtgaaatt
      181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      361 tgcgtattgc tctagaagcg gccgcgaatt ccaatggggt ctcgaggtcc tcgaagttgc
      421 aacgccgtcc ccacatcgtt gggtggtgac cggcaggtgt acaatgatac gaggtattga
      481 agattgaact aagtatactt tgcgcccgtt ctacatgaaa caaactcagt cgaacgatgc
      541 aagaacccgc attcggacca agttatgagc gcgaactccc gtttaaagct tttgtcgctc
      601 agaaacctga gggtttagca acgtttcacg tgggacatgc gaaccccctg ccaacgccgg
      661 agtatgggaa tgaacaaatc tgtcttcgtg ctaagggtgc ggttaggacg ctgaagtaac
      721 gcaacttata tgcaaggcaa attatagcag ggactaaatt ccagtaaggt gggagatcgt
      781 gacc
//
