LOCUS       sequence-137538-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter plasmids used in YeastFab work, in which promoters could be
            inserted just upstream YFP(YEVenus) to drive its expression, also 
            contains an mCherry gene driven by TEF2 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pYG026
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(106..411)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(208..227)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 accaaaattg ggacaacacc agtgaataat tcttcacctt tagacataaa aaccatctga
       61 gacccgtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
      121 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
      181 cccgataacg gagaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
      241 cccgatatgc accaccgggt aaagttcacg ggggacttta tctgacagca gacgtgcact
      301 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
      361 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
      421 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
      481 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
      541 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
      601 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg
      661 gtatacatat cagtatatat tcttataccg caa
//
