LOCUS       sequence-137550-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of
            transcriptional repression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
  TITLE     Optogenetic control of nuclear protein export.
  JOURNAL   Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
  PUBMED    26853913
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-8"; volume: "7:10624. doi"; pages: " 
            10.1038/ncomms10624"
COMMENT     Sequence Label: LEXY TR (with KRAB domain) (pDN163)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..80
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(247..441)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(790..843)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc
       61 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
      121 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
      181 ctccttgatg atggccatgt tatcctcctc gcccttgctc acgccgccgc tgccgccgct
      241 gccgccaaca agccatggtt cttccccctt ctccagccgc aggattacgt cgggttttgt
      301 gagttggtag cccaagctca caaggttttt gtagttctcc agcatcacgt ttctataaac
      361 gatctgctgg gcggtgtcca agagtttcca ctcctcccgg gtgaagtcca cgaacacatc
      421 tttgaaggtc accaaggttc ggctccaggc tgtcagagac tttgcgtcca tagagccgcc
      481 ggatcctccg ggttccccag cggctacgcg tcctacaaga ggaaggccct cctcttcctc
      541 ctgcagcaat cttatgcccc gagatgctcc tgagacaatc tcaatcacac cttttcgggc
      601 cagggccttc aagtgttcct ccgcagcatt cggggaccgg aatcccagcc gctgtgcaat
      661 ctctgccctc gtcgggggca tacctgtctg ggaaatatgg tctcgaatca ggtcaaagac
      721 ctcttgctgc cttgcagtaa gggccttcat actaccgccg tcgagcttaa ccctcttggc
      781 ggcggccccg ggaccagggt tctcctcaac atcgccacat gttagcaggc tccctcgacc
      841 ttcgccgcta ccaccaggca gcatgtccag atcaaa
//
