LOCUS       sequence-137553-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C. elegans let-858 3' UTR (Gateway Entry Clone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     David Tirrell's Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKPY738
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(30..153)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(564..659)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(696..715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(697..715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(720..736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggtcactaat accatctaag tagttggttc atagtgactg catatgttgt gttttacagt
       61 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
      121 acgtttctcg ttcaactttc ttgtacaaag tggccgctag ccggccatac aagtaatccg
      181 gatgatcgac gccaacgtcg ttgaattttc aaattttaaa tactgaatat ttgttttttt
      241 tcctattatt tatttattct ctttgtgttt tttttcttgc tttctaaaaa attaattcaa
      301 tccaaatcta aacatttttt tttctctttc cgtctcccaa ttcgtattcc gctcctctca
      361 tctgaacaca atgtgcaagt ttatttatct tctcgctttc atttcattag gacgtggggg
      421 gaattggtgg aagggggaaa cacacaaaag gatgatggaa atgaaataag gacacacaat
      481 atgcaacaac attcaattca gaaatatgga ggaaggttta aaagaaaaca taaaaatata
      541 tagaggagga aggaaaacta gtgcaacttt attatacaaa gttggcatta taaaaaagca
      601 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      661 gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg
      721 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      781 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaa
//
