LOCUS       sequence-137559-        6771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  green opsin promoter from chicken (Gallus gallus) gDNA 
            chr26:4,504,913–4,501,931 in galGal4 driving DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Gg 3kb Green opsin DsRed
FEATURES             Location/Qualifiers
     source          1..6771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3027..3036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3033..3710
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3213..3233)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     3625..3648
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(3795..3814)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3860..3915
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3914..3933)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4274..4290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4274..4290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4287..4309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4298..4314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4322..4328,4329..4346,4347..4352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4367..4388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4447..4642
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4493..4628
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4648..4782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4685..4704)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4739..4758
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4850..4867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5021..5609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5101..5120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5780..6571,6572..6640))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6403..6422
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6641..6745)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga attcctcgac ggtacctgtg acagggacac tgaaggaagc
       61 gaggttgcat tcaggacacg tcctggagga accagctaac ctccctggtg tcattttctt
      121 ctgcagagtt gattcgaggc cttcggcatt gctccggtgg ccgctgacct gcagagagct
      181 ctaaatccac atcgacaccg gcctcggggg attggccaaa acaccaacct cagcttgtga
      241 gggagaaagc tgggtcctcg aggacagctg agaagggaca gagccacgct cagaccctcc
      301 agcagcacac atgtggctcg ggcttacctg ctccatgttc agctccccag agtgggagaa
      361 gtccaggtca agtccaagtg aaagtcctct tggtcccact gttggcaatc ctgcgtgtga
      421 aaggtatctg ggtgccaagc atgaagccaa agtcatacca tgcattagct ggaatgtgga
      481 gaggagaaag tttgctctgg tcgaaggaaa acaaaacaaa aaaatgtgaa atcaataaat
      541 gatggtgatt ataccaataa taatcctact gatcctttcc cacagtatca gtgcacaatc
      601 tgaaatagac tttcatttta cctcctcctc cctcaaaccc gcttcctttg tacaactata
      661 ttacccttta gaagaatatg agacgctata aaatgtggca ggaagtctca caaagaaaca
      721 ctgggaggtg aaaatgaact ctcccatgag atatccatgt ttttatataa agctctggag
      781 aaaaaaacag atctttcata tgctgctggc acagacagca aagtccacgt cttttgaaga
      841 tggacaatcg gtgatgcttt ctatctgaag agttgccttc taccatagaa gttgggaatg
      901 gcacactgga attgaaattc acatctgcac gtgctttttt atatgcaaaa aaaacccatg
      961 ctcagctgag tcagaatcct gagcgtagtc aggaaaggca tgaaataggt gacagctttg
     1021 agaagtagag tttgtgtaga ctgcaccaca caaacctctt tccctcatgg aagatctcat
     1081 gtctaaagga gactcagact ggaaaattcc cagactagga atttcatgac tggcagtttt
     1141 gtgggatgtg cggggaaata ctcctgaaag gcacagacat cccatgggga tctcttatct
     1201 gagggcacct gaggctgctg tgacccactt actcttgcca tgtcctagtt ctgctcctct
     1261 gacctgtgca gatggatcag tgggccagcg attagggcat catccatcct acccacaccc
     1321 ctcagccttc tgctcctgcc ctctacctcc aaaccattcc agtcttgcag acctcattcc
     1381 tctagcaaga cactgttgac tttttccatc tatgttgcct tggtggaggg caacagtagg
     1441 aaggaggcta gcagcttacc gcattaacag atcagggcca ttaacaccac cagatgtcag
     1501 agattaatgg cagagcttca ctaaggcgcc ttaggcaggt ctcatggaga aggagcaccc
     1561 atgacccaga aggaagaaat gggaaagagc tttggcagga tagagcattg gtgctctgag
     1621 ctacagctga ccctgctgag aaggtttcat ggtcttgcgt gctctggaat gggaatccca
     1681 cactataaga tgtgtgctaa gagaccagga gaagtttatt tccaccccaa agtgcctaca
     1741 agctggtgct gctctctttg caccatctct ggaagaaatt caggacatca tcaaggtttc
     1801 ctttccttcc ccctctgcat tcatactctg cttctccagc aaactgcagt accttgacag
     1861 gctacttcta gtgttgtggc caaggaccct tcccaagccc tgctaatcct tgcaaaactg
     1921 caaccaaaac agctgcccag ctacctggga cgggaaggac atgcagatgg gagctgatca
     1981 tcaccacctc ctcgggagac cagaaggaag acagaagagg gtatgaagcc ctttgcactc
     2041 taatgcaatc agggtgcagc acagccgaac tgcccccaag gtgttctgtt aaccaagatg
     2101 gcagcctttg ggctggcagc agcttcccct cctgcctttg cccagtgccg ggcatgtagg
     2161 gggcccttac ccacacagga agagctcagt tgtgatggag acctacccaa accatgtggt
     2221 gctcacactg aatcaggcac ctgaagcaca gtgtggccaa actctctctg ctcttcccca
     2281 gcaacttgcc caagggcagt gaagcagagc tgggtactcc tcacacccca aacacttcaa
     2341 tggagtcctt cagccacaca gggatgtccc agaggctgtt atccaacatg gacttaagca
     2401 aaaacccatt tctcccatca gtcatccaaa ggacctactc cccacctcac cacctctctt
     2461 cctgccattg cccctcttcc aaagggctcc ccaagccctt cccttcctcc ccacaactcc
     2521 ttaaacaatg ttaaacactt attgccaatt aatacgcttt aatgcaggtt ttggggccaa
     2581 ctgactactt agagggtgct taaaccatca gcccctctca atcccgttgc ccttgtcata
     2641 caaacttagc ctagccccta atccggcctc cccccgctct ccctcaagga ggcccaaagg
     2701 tataaaacct ccctcggcac gcggcaccgc cggcccttgg cacggtgcgg ggccaacgca
     2761 ccacctctcc ttcccccggg atccactgct gttcttagtc ataaattcaa tttcggagct
     2821 gggggaggga tcttttggtg gggtatttat ttatttattt attaccgttg ggttggggat
     2881 tccctctcat ttttttttct ttcttttttt ttttttggta attggggttg aaacaaaatt
     2941 cattaccata gagcgcttga ttaaaaaaac ccttaaacgc aaagcagctg ccataatagg
     3001 taccgcgggc ccgggatcca ccggtcgcca ccatggcctc ctccgagaac gtcatcaccg
     3061 agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg
     3121 agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca
     3181 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
     3241 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
     3301 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggcgacc gtgacccagg
     3361 actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc
     3421 cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
     3481 tgtacccccg cgacggcgtg ctgaagggcg agacccacaa ggccctgaag ctgaaggacg
     3541 gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc
     3601 ccggctacta ctacgtggac gccaagctgg acatcacctc ccacaacgag gactacacca
     3661 tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag cggccgcact
     3721 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     3781 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     3841 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     3901 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     3961 gagtatttgg tttagagttt ggcaacatat gccatatgct ggctgccatg aacaaaggtg
     4021 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     4081 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     4141 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     4201 actcccagtc atagctgtcc ctcttctctt atgaagatcc ctcgacctgc agcccaagct
     4261 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4321 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4381 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4441 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4501 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4561 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4621 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     4681 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4741 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     4801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     5221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5461 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
