LOCUS       sequence-137560-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receiving vector of terminators for the construction of standard 
            terminator parts in YeastFab work.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HcKan_T
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             672..683
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    701..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag
       61 gtgatgtcca gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg ttttttagcc
      121 atgtaggtgg ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt
      181 ttgatttcac ctttcagagc accgtcttcc gggtacatac gttcggtgga agcttcccaa
      241 cccatggttt ttttctgcat aaccggaccg tcggacggga agttggtacc acgcagttta
      301 actttgtaga tgaactcacc gtcttgcagg gaggagtcct gggtaacggt aacaacacca
      361 ccgtcttcga agttcataac acgttcccat ttgaaacctt ccgggaagga cagtttcagg
      421 tagtccggga tgtcagccgg gtgtttaacg taagctttgg aaccgtactg gaactgcggg
      481 gacaggatgt cccaagcgaa cggcagcgga ccacctttgg taactttcag tttagcggtc
      541 tgggtacctt cgtacggacg accttcacct tcaccttcga tttcgaactc gtgaccgtta
      601 acggaacctt ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcggaggaa
      661 gccatctagt atttctcctc tttctctagt atgtgtgaaa ttgttatccg ctcacaattc
      721 cacacaacat acgagccgga agcataa
//
