LOCUS       sequence-137561-        6815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  green opsin promoter from chicken (Gallus gallus) gDNA 
            chr26:4,504,913–4,501,931 in galGal4 driving GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 6815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Gg 3kb Green opsin GFP
FEATURES             Location/Qualifiers
     source          1..6815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3027..3036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3033..3035,3036..3038,3039..3752)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3033..3035,3036..3038,3039..3749)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3078..3099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3339..3358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3686..3707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3839..3858)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3904..3959
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3958..3977)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4318..4334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4318..4334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4331..4353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4342..4358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4366..4372,4373..4390,4391..4396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4411..4432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4491..4686
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4537..4672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4692..4826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4729..4748)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4783..4802
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4894..4911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5065..5653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5145..5164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5824..6615,6616..6684))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6447..6466
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6685..6789)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga attcctcgac ggtacctgtg acagggacac tgaaggaagc
       61 gaggttgcat tcaggacacg tcctggagga accagctaac ctccctggtg tcattttctt
      121 ctgcagagtt gattcgaggc cttcggcatt gctccggtgg ccgctgacct gcagagagct
      181 ctaaatccac atcgacaccg gcctcggggg attggccaaa acaccaacct cagcttgtga
      241 gggagaaagc tgggtcctcg aggacagctg agaagggaca gagccacgct cagaccctcc
      301 agcagcacac atgtggctcg ggcttacctg ctccatgttc agctccccag agtgggagaa
      361 gtccaggtca agtccaagtg aaagtcctct tggtcccact gttggcaatc ctgcgtgtga
      421 aaggtatctg ggtgccaagc atgaagccaa agtcatacca tgcattagct ggaatgtgga
      481 gaggagaaag tttgctctgg tcgaaggaaa acaaaacaaa aaaatgtgaa atcaataaat
      541 gatggtgatt ataccaataa taatcctact gatcctttcc cacagtatca gtgcacaatc
      601 tgaaatagac tttcatttta cctcctcctc cctcaaaccc gcttcctttg tacaactata
      661 ttacccttta gaagaatatg agacgctata aaatgtggca ggaagtctca caaagaaaca
      721 ctgggaggtg aaaatgaact ctcccatgag atatccatgt ttttatataa agctctggag
      781 aaaaaaacag atctttcata tgctgctggc acagacagca aagtccacgt cttttgaaga
      841 tggacaatcg gtgatgcttt ctatctgaag agttgccttc taccatagaa gttgggaatg
      901 gcacactgga attgaaattc acatctgcac gtgctttttt atatgcaaaa aaaaaccatg
      961 ctcagctgag tcagaatcct gagcgtagtc aggaaaggca tgaaataggt gacagctttg
     1021 agaagtagag tttgtgtaga ctgcaccaca caaacctctt tccctcatgg aagatctcat
     1081 gtctaaagga gactcagact ggaaaattcc cagactagga atttcatgac tggcagtttt
     1141 gtgggatgtg cggggaaata ctcctgaaag gcacagacat cccatgggga tctcttatct
     1201 gagggcacct gaggctgctg tgacccactt actcttgcca tgtcctagtt ctgctcctct
     1261 gacctgtgca gatggatcag tgggccagcg attagggcat catccatcct acccacaccc
     1321 ctcagccttc tgctcctgcc ctctacctcc aaaccattcc agtcttgcag acctcattcc
     1381 tctagcaaga cactgttgac tttttccatc tatgttgcct tggtggaggg caacagtagg
     1441 aaggaggcta gcagcttacc gcattaacag atcagggcca ttaacaccac cagatgtcag
     1501 agattaatgg cagagcttca ctaaggcgcc ttaggcaggt ctcatggaga aggagcaccc
     1561 atgacccaga aggaagaaat gggaaagagc tttggcagga tagagcattg gtgctctgag
     1621 ctacagctga ccctgctgag aaggtttcat ggtcttgcgt gctctggaat gggaatccca
     1681 cactataaga tgtgtgctaa gagaccagga gaagtttatt tccaccccaa agtgcctaca
     1741 agctggtgct gctctctttg caccatctct ggaagaaatt caggacatca tcaaggtttc
     1801 ctttccttcc ccctctgcat tcatactctg cttctccagc aaactgcagt accttgacag
     1861 gctacttcta gtgttgtggc caaggaccct tcccaagccc tgctaatcct tgcaaaactg
     1921 caaccaaaac agctgcccag ctacctggga cgggaaggac atgcagatgg gagctgatca
     1981 tcaccacctc ctcgggagac cagaaggaag acagaagagg gtatgaagcc ctttgcactc
     2041 taatgcaatc agggtgcagc acagccgaac tgcccccaag gtgttctgtt aaccaagatg
     2101 gcagcctttg ggctggcagc agcttcccct cctgcctttg cccagtgccg ggcatgtagg
     2161 gggcccttac ccacacagga agagctcagt tgtgatggag acctacccaa accatgtggt
     2221 gctcacactg aatcaggcac ctgaagcaca gtgtggccaa actctctctg ctcttcccca
     2281 gcaacttgcc caagggcagt gaagcagagc tgggtactcc tcacacccca aacacttcaa
     2341 tggagtcctt cagccacaca gggatgtccc agaggctgtt atccaacatg gacttaagca
     2401 aaaacccatt tctcccatca gtcatccaaa ggacctactc cccacctcac cacctctctt
     2461 cctgccattg cccctcttcc aaagggctcc ccaagccctt cccttcctcc ccacaactcc
     2521 ttaaacaatg ttaaacactt attgccaatt aatacgcttt aatgcaggtt ttggggccaa
     2581 ctgactactt agagggtgct taaaccatca gcccctctca atcccgttgc ccttgtcata
     2641 caaacttagc ctagccccta atccggcctc cccccgctct ccctcaagga ggcccaaagg
     2701 tataaaacct ccctcggcac gcggcaccgc cggcccttgg cacggtgcgg ggccaacgca
     2761 ccacctctcc ttcccccggg atccactgct gttcttagtc ataaattcaa tttcggagct
     2821 gggggaggga tcttttggtg gggtatttat ttatttattt attaccgttg ggttggggat
     2881 tccctctcat ttttttttct ttcttttttt ttttttggta attggggttg aaacaaaatt
     2941 cattaccata gagcgcttga ttaaaaaaac ccttaaacgc aaagcagctg ccataatagg
     3001 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
     3061 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     3121 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     3181 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     3241 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     3301 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     3361 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     3421 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     3481 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     3541 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     3601 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     3661 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3721 tcactctcgg catggacgag ctgtacaagt aaagcggccg cactcctcag gtgcaggctg
     3781 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     3841 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     3901 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     3961 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     4021 gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat
     4081 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     4141 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     4201 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     4261 gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     4321 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4381 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     4501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     4861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     5281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     5521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6781 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
