LOCUS       sequence-137562-        5774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  violet opsin promoter from zebra finch (Taeniopygia guttata) gDNA 
            (nucleotides21,946 to 145, corresponding to 
            chr1A_ran-dom:206453–208444 in taeGut1) driving DsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Enright JM, Lawrence KA, Hadzic T, Corbo JC
  TITLE     Transcriptome profiling of developing photoreceptor subtypes reveals
            candidate genes involved in avian photoreceptor diversification.
  JOURNAL   J Comp Neurol. 2015 Mar 1;523(4):649-68. doi: 10.1002/cne.23702. 
            Epub 2014 Dec 1.
  PUBMED    25349106
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cne.23702";
            journalName: "J Comp Neurol"; date: "2015-03-1- 1"; volume: "523"; 
            issue: "4"; pages: "649-68"
COMMENT     Sequence Label: Tg 2 kb Violet opsin DsRed
FEATURES             Location/Qualifiers
     source          1..5774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1970..1979
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2030..2039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2036..2713
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2216..2236)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2628..2651
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2798..2817)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2863..2918
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2917..2936)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3277..3293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3277..3293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3290..3312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3301..3317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3325..3331,3332..3349,3350..3355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3370..3391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3450..3645
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3496..3631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3651..3785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3688..3707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3742..3761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3853..3870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4024..4612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4104..4123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4783..5574,5575..5643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5406..5425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5644..5748)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacgata tctctagaga attcctcgac ggtaccagtg ggtgctgggg gcttggtggt
       61 gggcgctggt gggtgctggg gttcctggca gggcagtgcc accgctgcag cccccagagc
      121 tccatgccca gcatcccccg agtcccttcc ctgctccatc cgcaatccca gaaccacaga
      181 accacagaac ccccgaggct ggaaaagccc tcccggccat ggagtctaag ctgtgcctga
      241 ctcctccttg tcccggagcc ctgggtgcca cctccaggcc tcccttggac acctgtgggg
      301 acgggcaccc caaacctccc cgggcagccc ctgccgaggc tgacagctcc gtcgtgccct
      361 ggctctgcca tcccccacag cccctgcgct gtgtcccccc tccgcagctc acagtgccca
      421 cagtgcccac agtgaccgca gctccacggg ggacacggcg gagggaccct ttgtggggtg
      481 tctgtcccct gggtcaccac cctggggcac ccatttgggg gtgcccctcc atgggacgcc
      541 cagtagagca ccagcagagc tgaccctacc cggtacagcc cagtacagcc cagtacaacc
      601 cagtacggcc cagtagagcc cagtagagcc tgctacagta tgaccaaacc cagtacaacc
      661 caacagggca cagtgcaatc ccatggcaca gtacagtgca gtacagccca gtgtagtcca
      721 gtacagccca gtacaaacca gtacagtaca ggccaatatg atccaataca gcccgtaaaa
      781 catgggctgg gggttcagcc cacccaatcc ccctgttcct cccctggccc catttctggc
      841 cgtccctgag cccccctggc cccatctctg ccccagagct ggacagaagc cgagggtgcc
      901 tcaggacacc ccagggtggg caccccacac acacccaggg cacagcgttc cctgaaggtg
      961 tccccctccc cacaaatgag atccccctgc cctgggggtc ccggggtgcc acagtgaggt
     1021 ccctgcacca gcctttatag gcacccccag cccggggggg ccctgtaatc cccccctaag
     1081 cagcttctgc tcatccttcc tgttccccgg gggtgccaca ggctggggga gggaaagagg
     1141 ggggagggag ggcaggaaac gctgggagtc ttctgggact cgagtcccag cagcacaccc
     1201 ccaaagtgac cctccatcaa tgaccccctg gcactgcacc cccaaaatga acaccccctc
     1261 tcagacaccc cacctcaagc agtgccccct cagtcttccc cccacccctt gataccccca
     1321 gacaccacca cctcacttgg ggtctcccca gcacagggat gccccaagcc cagaactggg
     1381 gacaatcgct tgaaggggga gacacccggg gggcaccctg gggtgctggg gtggggggca
     1441 gcaggtgagc tcatgaggaa actccgagtt cttcacaaag catccagggc cggggcaccc
     1501 tgggggagtg ggatcaaggc tggagacacc tggggacccc tggggatacc tagggacacc
     1561 tggccattct gggggtgacc aggacacagc ttggggaccc ctggggcaca ctgaggaccc
     1621 tggtgggatc aggactaagg ctggggacct ctagctgttg tggtgggacc aagacttggg
     1681 cttgggaaca cctggggaca cctgaacacc tcaggaggag caggatcaag cttagagtcc
     1741 cctggggacc ctccccatac ccagatgttg gggggagccc ttctgccccg ccccccaaat
     1801 cctccagagc accggcaatc ggctcgaccc ggctcagggg gccgattagg ggctgagccc
     1861 agtttagccc accctaatcc ccaagtttgg ccgggctgag ccctgatccc ccctaagcag
     1921 ctttgggggc gcagggtata tcaggggggt gggcgccccc cccgccagcg ccaccatgga
     1981 cgaggaagag ttttacctgt tcaagaacca gtcgtcggtg agaccggtcg ccaccatggc
     2041 ctcctccgag aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt
     2101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa
     2161 caccgtgaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     2221 cccccagttc cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta
     2281 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     2341 cggcgtggcg accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt
     2401 gaagttcatc ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg
     2461 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca
     2521 caaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat
     2581 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac
     2641 ctcccacaac gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca
     2701 cctgttcctg tagcggccgc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
     2761 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
     2821 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     2881 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     2941 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat
     3001 gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct
     3061 gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt
     3121 tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc
     3181 agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga
     3241 tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3421 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     3661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3781 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4201 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4441 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     4501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5761 aaaagtgcca cctg
//
