LOCUS       sequence-137563-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of TEF2 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-TEF2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(28..130)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(131..162)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 agacgtttcc cgttgaatat ggctcatttt agcttcctta gctcctgaaa atctcgataa
       61 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
      121 gtgccgatca agtcaaaagc ctccggtcgg aggcttttga ctttctgcta tggaggtcag
      181 gtatgattta aatggtcagt cgtctcgcat ctttagttaa ttatagttcg ttgaccgcat
      241 attctaaaaa caagtactcc ttaaaaaaaa accttgaagg gaataaacaa gtagaataga
      301 tagagagaaa aatagaaaat gcaagagaat ttatatatta gaaagagaga aagaaaaatg
      361 gaaaaaaaaa aataggaaaa gccagaaata gcactagaag gagcgacacc agaaaagaag
      421 gtgatggaac caatttagct atatatagtt aactaccggc tcgatcatct ctgcctccag
      481 catagtcgaa gaagaatttt ttttttcttg aggcttctgt cagcaactcg tattttttct
      541 tccttttttg gtgagcctaa aaagttccca cgttctcttg tacgacgccg tcacaaacaa
      601 ccttatgggt aatttgtcgc ggtctgggtg tataaatgtg tgggtgcaac atgaatgtac
      661 ggaggtagtt tgctgattgg cggtctatag ataccttggt tatggcgccc agcccgagac
      721 ggactccagc gtaactggac tgcaatcaac tcactggctc accttcacgg
//
