LOCUS       sequence-137565-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  construction of ADH1 terminator in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-T-ADH1
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(27..129)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(130..161)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     terminator      complement(211..397)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     319..343
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      443..472
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gacgtttccc gttgaatatg gctcatttta gcttccttag ctcctgaaaa tctcgataac
       61 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      121 tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat ggaggtcagg
      181 tatgatttaa atggtcagtc gtctcggagg ccggtagagg tgtggtcaat aagagcgacc
      241 tcatgctata cctgagaaag caacctgacc tacaggaaag agttactcaa gaataagaat
      301 tttcgtttta aaacctaaga gtcactttaa aatttgtata cacttatttt ttttataact
      361 tatttaataa taaaaatcat aaatcataag aaattcggct acgagacgga ctccagcgta
      421 actggactgc aatcaactca ctggctcacc ttcacgggtg ggcctttctt cggtagaaaa
      481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      781 gttaccggat aaggcgcagc ggtcgggctg aacgg
//
