LOCUS       sequence-137571-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for 
            sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Schwartz CM, Hussain MS, Blenner M, Wheeldon I
  TITLE     Synthetic RNA polymerase III promoters facilitate high efficiency 
            CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2015 Dec 29.
  PUBMED    26714206
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 29."
COMMENT     Sequence Label: pCRISPRyl
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaacgcgcga gacgaaaggg ccagatctgt tcggaaatca acggatgctc aaccgatttc
       61 gacagtaata atttgaatcg aatcggagcc taaaatgaac ccgagtatat ctcataaaat
      121 tctcggtgag aggtctgtga ctgtcagtac aaggtgcctt cattatgccc tcaaccttac
      181 catacctcac tggatgtagt gtacctctaa aaatgaaata cagtgccaaa agccatggca
      241 ctgagctcgt ctaacggact tgatatacaa ccaattaaaa caaatgaaaa gaaatacagt
      301 tctttgtatc atttgtaaca attaccctgt acaaactaag gtattgaaat cccacaatat
      361 tcccaaagtc cacccctttc caaattgtca tgcctacaac tcatatacca gacactaacc
      421 taccaaacac cactaaaaac cccacaaaat atatcttacc gaatatacag taacaagcta
      481 ccaccacact cgttgggtgc agtcgccagc tttaaagata tctatccaca tcagccacaa
      541 ctcccttcct ttaataaacc gactacaccc ttggctattg aggttatgag tgaatatact
      601 gtagacaaga cactttcaag aagactgttt ccaaaacgta ccactgtcct ccactacaaa
      661 cacacccaat ctgcttcttc tagtcaaggt tgctacaccg gtaaattata aatcatcatt
      721 tcattagcag ggctgggccc tttttataga gtcttataca ctagcggacc ctgccggtag
      781 accaacccgc aggcgcgtca gtttgctcct tccatcaatg cgtcgtagaa acgacttact
      841 ccttcttgag cagctccttg accttgttgg caacaaagtc tccgacctcg gaggtggagg
      901 aagagcctcc gatatcggcg gtagtgatac cagcctcgac ggactccttg acggcagcct
      961 caacagcgtc accggcgggc ttcatgttaa gagagaactt gagcatcatg gcggcagaca
     1021 gaatggtggc aatgggg
//
