LOCUS       sequence-137577-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for 
            sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Schwartz CM, Hussain MS, Blenner M, Wheeldon I
  TITLE     Synthetic RNA polymerase III promoters facilitate high efficiency 
            CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2015 Dec 29.
  PUBMED    26714206
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 29."
COMMENT     Sequence Label: pCRISPRyl
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcctccagtc ggtggaagaa agagtcgtcc accttggcca tctcgttaga gaagatctcc
       61 tgcaggtaac agattcggtt ctttcttcgg gtgtatcgtc ttcgagcggt tcgcttcagt
      121 cgggtggcct cagcggtctc gccagagtcg aagagcagag cgccgatcag gttcttcttg
      181 atggagtgtc ggtcggtgtt gcccaggacc ttgaatttct tagagggcac cttgtactca
      241 tcggtgatca cggcccaacc cacggagttg gttccgatgt ccaggccaat ggagtatttc
      301 ttatccattg tggcgcgcct ttgaatgatt cttatactca gaaggaaatg cttaacgatt
      361 tcgggtgtga gttgacaagg agagagagaa aagaagagga aaggtaattc ggggacggtg
      421 gtcttttata cccttggcta aagtcccaac cacaaaagct tctgcaggca tgcccggatc
      481 gaggtgggcg ggaacaacgg tctcgataat gtacgtacac tacacacaaa gagaagcaag
      541 tgggggcggg actgcacggc acttgtgaga cacctcaggt cgacccggat cgaggtgggc
      601 gggaacaacg gtctcgataa tgtacgtaca ctacacacaa agagaagcaa gtgggggcgg
      661 gactgcacgg cacttgtgag acacctcagg gatccccgga tcgaggtggg cgggaacaac
      721 ggtctcgata atgtacgtac actacacaca aagagaagca agtgggggcg ggactgcacg
      781 gcacttgtga gacacctcag tctagaccgg atcgaggtgg gcgggaacaa cggtctcgat
      841 aatgtacgta cactacacac aaagagaagc aagtgggggc gggactgcac ggcacttgtg
      901 agacacctca ggagctcccg gatcgaggtg ggcgggaaca acggtctcga taatgtacgt
      961 acactacaca caaagagaag caagtggggg cgggactgca cggcacttgt gagacacctc
     1021 aggtcgaccc ggatcgaggt gggcggg
//
