LOCUS       sequence-137579-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pbuE Sense Expression Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Chappell J, Takahashi MK, Lucks JB
  TITLE     Creating small transcription activating RNAs.
  JOURNAL   Nat Chem Biol. 2015 Mar;11(3):214-20. doi: 10.1038/nchembio.1737. 
            Epub 2015 Feb 2.
  PUBMED    25643173
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1737"; journalName: "Nat Chem Biol"; date: 
            "2015-03"; volume: "11"; issue: "3"; pages: "214-20"
COMMENT     Sequence Label: pJBL2184
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..220
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     complement(344..372)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
       61 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
      121 ataagcttcc gatggcgcgc cgagaggctt tacactttat gcttccggct gaattctaaa
      181 gatctttgac agctagctca gtcctaggta taatactagt gtgtctacca ggaaccgtaa
      241 aatcctgatt acaaaatttg tttatgacat tttttgtaat caggattttt tttggatcta
      301 ggaggaagga tctatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt
      361 tgaattagat ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga
      421 tgctacaaac ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc
      481 gtggccaaca cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga
      541 tcacatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg
      601 cactatatct ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg
      661 tgataccctt gttaatcgta tcgagttaaa gggtattgat tttaaagaag atggaaacat
      721 tcttggacac aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa
      781 acaaaagaat ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt
      841 tcaactagca gaccattatc aacaaaa
//
