LOCUS       sequence-137580-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of mCherry, TVA and RabG in human and mouse neurons 
            for rabies mono-synaptic transfer experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     Maletic-Savatic LAB, unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSynI-mCherry-F2A-TVA-T2A-RabG-WPRE-hGH p...
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(204..222)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     472..491
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgtcgtgcc tgagagcgca gtcgagaagg taccggatcc tctagagtcg acgccaccat
       61 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      121 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      181 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      241 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      301 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      361 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      421 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      481 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      541 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      601 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      661 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      721 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggta gtggtgtgaa
      781 acagactttg aatttt
//
