LOCUS       sequence-137582-        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of mCherry, TVA and RabG in human and mouse neurons 
            for rabies mono-synaptic transfer experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  TITLE     Maletic-Savatic LAB, unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSynI-mCherry-F2A-TVA-T2A-RabG-WPRE-hGH p...
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             539..592
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gactttgaat tttgaccttc tcaagttggc gggagacgtg gagtccaacc cagggcccat
       61 ggcgcggctg ctgcccgcgc tgctgctgct gctgctgccc ggtaacgtga ccggtaacgg
      121 gtccggtaac ggttctttgt cccgttgccc ccccggtcag ttccgctgct cggagccgcc
      181 cggtgcccac ggggagtgtt acccgcagga ctggctgtgc gacggacacc ccgactgcga
      241 cgacgggcgg gacgagtggg gctgcgggac cagcgcgatc cccgcggtgc ccacggacaa
      301 cggcacagag gctcccactg cccctgctcc tggacgtgct ctgccagcca ggaatcacgg
      361 ccgcatgtgg atgctgatca ctgcagtgct cctgtgctgc ctggtagctg tgggtggtat
      421 cgctgcatgg gggaagtcca aagcaaaaag caggtctgac atcttcagtc ttgaaagcgc
      481 atccaaggag ctgctggtgc ctgacaagag ccaggcagac ttgttctccg gtagtggaga
      541 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caatggttcc
      601 tcaggctctc ctgtttgtac cccttctggt ttttccattg tgttttggga aattccctat
      661 ttacacgata ccagacaagc ttggtccctg gagtccgatt gacatacatc acctcagctg
      721 cccaaacaat ttggtagtgg aggacgaagg atgcaccaac ctgtcagggt tctcctacat
      781 ggaacttaaa gttggataca tcttagccat aaaagtgaac gggttcactt gcacaggcgt
      841 tgtgacggag gctgaaacct acactaactt cgttggttat gtcacaacca cgttcaaaag
      901 aaagcatttc cgcccaacac cagatgcatg tagagccgcg tacaactgga agatggccgg
      961 tgaccccaga tatgaagagt ctctacacaa tccgtaccct gactaccgct ggcttcgaac
     1021 tgtaaaaacc accaaggagt ctctcgttat catatctcca agtgtggcag atttggaccc
     1081 atatgacaga tcccttcact cgagggtctt ccctagcggg aagtgctcag ga
//
