LOCUS       sequence-137583-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing human codon-optimized S. pyogenes 
            FLAG-tagged Cas9 and blasticidin resistance from EFS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.11.015"; journalName: "Cell"; date: "2015-12-3-
            3"; volume: "163"; issue: "6"; pages: "1515-26"
COMMENT     Sequence Label: Lenti‐Cas9‐2A‐Blast
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccagttcgct caggccgcct ctctcggcct tggtcagatt gtcgaacttt ctctgggtaa
       61 tcagcttggc gttcagcagc tgccgccagt agttcttcat cttcttcacg acctcttcgg
      121 agggcacgtt gtcgctcttg ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga
      181 tggagtcgtc cttcagaaag ctctgaggca cgatatggtc cacatcgtag tcggacagcc
      241 ggttgatgtc cagttcctgg tccacgtaca tatcccgccc attctgcagg tagtacaggt
      301 acagcttctc gttctgcagc tgggtgtttt ccacggggtg ttctttcagg atctggctgc
      361 ccagctcttt gatgccctct tcgatccgct tcattctctc gcggctgttc ttctgtccct
      421 tctgggtggt ctggttctct ctggccattt cgatcacgat gttctcgggc ttgtgccggc
      481 ccatcacttt cacgagctcg tccaccacct tcactgtctg caggatgccc ttcttaatgg
      541 cggggctgcc ggccagattg gcaatgtgct cgtgcaggct atcgccctgg ccggacacct
      601 gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc gtggatcagc tgcatgaagt
      661 ttctgttggc gaagccgtcg gacttcagga aatccaggat tgtcttgccg gactgcttgt
      721 cccggatgcc gttgatcagc ttccggctca gcctgcccca gccggtgtat ctccgccgct
      781 tcagctgctt catcactttg tcgtcgaaca ggtgggcata ggttttcagc cgttcctcga
      841 tcatctctct gtcctcaaac agtgtcaggg tcagcacgat atcttccaga atgtcctcgt
      901 tttcctcatt gtccaggaag tccttgtcct tgataatttt cagcagatcg tggtatgtgc
      961 cca
//
