LOCUS       sequence-137584-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing human codon-optimized S. pyogenes 
            FLAG-tagged Cas9 and blasticidin resistance from EFS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.11.015"; journalName: "Cell"; date: "2015-12-3-
            3"; volume: "163"; issue: "6"; pages: "1515-26"
COMMENT     Sequence Label: Lenti‐Cas9‐2A‐Blast
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..452)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(453..506)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(522..569)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttaacg cgtttagccc
       61 tcccacacat aaccagaggg cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca
      121 ctgtccttca ctatggcttt gatcccagga tgcagatcga gaagcacctg tcggcaccgt
      181 ccgcaggggc tcaagatgcc cctgttctca tttccgatcg cgacgataca agtcaggttg
      241 ccagctgccg cagcagcagc agtgcccagc accacgagtt ctgcacaagg tcccccagta
      301 aaatgatata cattgacacc agtgaagatg cggccgtcgc tagagagagc tgcgctggcg
      361 acgctgtagt cttcagagat ggggatgctg ttgattgtag ccgttgctct ttcaatgagg
      421 gtggattctt cttgagacaa aggcttggcc attgggcctg gattttcttc gacatcaccg
      481 catgttagca gacttcctct gccctcgccg ctgccgctag ctttcttttt cttagcttga
      541 ccagctttct tagtagcagc aggacgcttg tcgcctccca gctgagacag gtcgatccgt
      601 gtctcgtaca ggccggtgat gctctggtgg atcagggtgg cgtccagcac ctctttggtg
      661 ctggtgtacc tcttccggtc gatggtggtg tcaaagtact tgaaggcggc aggggctccc
      721 agattggtca gggtaaacag gtggatgata ttctcggcct gctctctgat gggcttatcc
      781 cggtgcttgt tgtaggcgga cagcactttg tccagattag cgtcggccag gatcactctc
      841 ttggagaact cgctgatctg ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca
      901 aacagctgtt tctgctcatt atcctcgggg
//
