LOCUS       sequence-137585-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing human codon-optimized S. pyogenes 
            FLAG-tagged Cas9 and blasticidin resistance from EFS promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod 
            G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, 
            Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
  TITLE     High-Resolution CRISPR Screens Reveal Fitness Genes and 
            Genotype-Specific Cancer Liabilities.
  JOURNAL   Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. 
            Epub 2015 Nov 25.
  PUBMED    26627737
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.11.015"; journalName: "Cell"; date: "2015-12-3-
            3"; volume: "163"; issue: "6"; pages: "1515-26"
COMMENT     Sequence Label: Lenti‐Cas9‐2A‐Blast
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    209..326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(527..547)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(725..769)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
       61 ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt
      121 gggcgatgtg cgctctgccc actgacgggc accggagcca ctcgagccgg cgccaaagtg
      181 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
      241 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
      301 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctcaagatct
      361 agaatttatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
      421 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
      481 taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
      541 ggccctctct gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
      601 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
      661 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
      721 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
      781 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
      841 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
      901 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
      961 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     1021 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cc
//
