LOCUS       sequence-137588-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid to generate guideRNA against mmp21 gene in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Perles Z, Moon S, Ta-Shma A, Yaacov B, Francescatto L, Edvardson S, 
            Rein AJ, Elpeleg O, Katsanis N
  TITLE     A human laterality disorder caused by a homozygous deleterious 
            mutation in MMP21.
  JOURNAL   J Med Genet. 2015 Dec;52(12):840-7. doi: 
            10.1136/jmedgenet-2015-103336. Epub 2015 Oct 1.
  PUBMED    26429889
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1136/jmedgenet-2015-103336"; journalName: "J Med Genet"; date: 
            "2015-12"; volume: "52"; issue: "12"; pages: "840-7"
COMMENT     Sequence Label: pT7-gRNA_zebrafish_mmp21
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(8..83)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(102..120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(144..160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(157..179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    168..184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(192..198,199..216,217..222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    237..258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(375..392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(626..645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggatccgca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
       61 cttgctattt ctagctctaa aactgtcagt gatcagctcc gcctatagtg agtcgtatta
      121 atttcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      181 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      241 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      301 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1081 ggtggttttt
//
