LOCUS       sequence-137589-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid to generate guideRNA against mmp21 gene in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Perles Z, Moon S, Ta-Shma A, Yaacov B, Francescatto L, Edvardson S, 
            Rein AJ, Elpeleg O, Katsanis N
  TITLE     A human laterality disorder caused by a homozygous deleterious 
            mutation in MMP21.
  JOURNAL   J Med Genet. 2015 Dec;52(12):840-7. doi: 
            10.1136/jmedgenet-2015-103336. Epub 2015 Oct 1.
  PUBMED    26429889
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1136/jmedgenet-2015-103336"; journalName: "J Med Genet"; date: 
            "2015-12"; volume: "52"; issue: "12"; pages: "840-7"
COMMENT     Sequence Label: pT7-gRNA_zebrafish_mmp21
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..24
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        43..118
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(146..163)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(146..162)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(155..177)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        382..486
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(705..724)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tgaattaata cgactcacta taggcggagc tgatcactga cagttttaga gctagaaata
       61 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcgg
      121 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      181 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      241 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      301 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaggt
      361 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      421 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      481 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      541 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      601 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      661 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      721 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      781 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      841 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      901 acgatcggag gaccgaagga gctaaccgct tttt
//
