LOCUS       sequence-13760-e        9422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Paz Z, Garcia-Pedrajas MD, Andrews DL, Klosterman SJ, Baeza-Montanez
            L, Gold SE
  TITLE     One step construction of Agrobacterium-Recombination-ready-plasmids 
            (OSCAR), an efficient and robust tool for ATMT based gene deletion 
            construction in fungi.
  JOURNAL   Fungal Genet Biol. 2011 Jul;48(7):677-84. doi: 
            10.1016/j.fgb.2011.02.003. Epub 2011 Mar 6.
  PUBMED    21362493
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fgb.2011.02.003"; journalName: "Fungal Genet Biol"; date:
            "2011-07"; volume: "48"; issue: "7"; pages: "677-84"
COMMENT     Sequence Label: pOSCAR
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1742..1766
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1829..1845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1829..1845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1850..1869
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1850..1868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1906..2137
                     /gene="mutant version of attP"
                     /label=attP3
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2494..2799)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2596..2615)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3141..3800)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3715..3734
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3801..3903)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    4061..4292
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4330..4346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4629..4653
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5181..5972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6216..6804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6705..6724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6958..6975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6990..7130
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7056..7075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7216..7238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7474..7668
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7734..8801)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(join(9235..9422,1..442))
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRNGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
       61 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
      121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
      181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
      241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
      301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
      361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
      421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
      481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
      541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
      601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
      661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
      721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
      781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
      841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
      901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
      961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
     1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
     1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
     1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
     1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
     1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
     1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
     1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
     1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
     1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
     1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
     1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta
     1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
     1801 caatctgatc caagctcaag ctaagcttca ggaaacagct atgaccatgt aatacgactc
     1861 actatagggg atatcgcggc cgcgttaacg ctaccatgga gctccaaata atgattttat
     1921 tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt tttataatgc
     1981 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     2041 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac
     2101 tatgaatcaa ctacttagat ggtattagtg acctgtagaa ttcgagctcg accgacagcc
     2161 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat
     2221 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac
     2281 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca
     2341 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga
     2401 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc
     2461 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
     2521 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     2581 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     2641 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     2701 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     2761 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt
     2821 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     2881 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     2941 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     3001 cactgtaata cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca
     3061 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
     3121 agtaagccgg atccacgcga ttacgccccg ccctgccact catcgcagta ctgttgtaat
     3181 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc
     3241 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     3301 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     3361 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     3421 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     3481 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     3541 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     3601 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     3661 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     3721 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     3781 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     3841 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     3901 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     3961 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg
     4021 tcgggtgatg ctgccaactt agtcgacgct ctagagctcc aaataatgat tttattttga
     4081 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact
     4141 ttgtacaaga aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     4201 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatgca gtcactatga
     4261 atcaactact tagatggtat tagtgacctg tagaattcga gctctagagc tgcagggccc
     4321 gagcttaaga ctggccgtcg ttttacggta ccgcgatcgc tcgcgacctg caggcataaa
     4381 gccgtcagtg tccgcataaa gaaccaccca taatacccat aatagctgtt tgccatcgct
     4441 accttaggac cgttatagtt aattaccctg ttatccctat taattaagag ctcgctacct
     4501 taagagagga tatcggcgcg cctctagaat ttaaatggat cctacgtact cgaggaattc
     4561 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     4621 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     4681 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     4741 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     4801 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     4861 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     4921 ctcgttcggt tcgtaagctg taatgcaagt agcgtaactg ccgtcacgca actggtccag
     4981 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttcttgtta
     5041 tgacatgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     5101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     5161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     5221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     5281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     5341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     5401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     5461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     5521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     5581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     5641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     5701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     5761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     5821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     5881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     5941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     6001 cgccgcttcg ccggcgttaa ctcaagcgat tagatgcact aagcacataa ttgctcacag
     6061 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
     6121 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga
     6181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6361 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6961 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7021 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7081 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     7141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7261 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
     7321 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
     7381 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
     7441 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
     7501 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
     7561 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
     7621 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
     7681 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
     7741 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
     7801 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
     7861 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
     7921 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
     7981 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
     8041 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
     8101 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
     8161 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
     8221 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
     8281 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
     8341 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
     8401 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
     8461 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
     8521 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
     8581 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
     8641 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
     8701 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
     8761 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg
     8821 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca
     8881 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt
     8941 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt
     9001 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta
     9061 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca
     9121 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac
     9181 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat
     9241 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg
     9301 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt
     9361 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc
     9421 gt
//
