LOCUS       sequence-137612-       10264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p306-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..10264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        196..411
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             412..1215
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1346..1801)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1483..1504)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1695..1714
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1931..1953
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1945..1962
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1946..1962
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1969..1988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1969..1987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3466..4169)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     4958..4974
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4958..4974
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        5071..5714
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5607..5630
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5733..6863
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLERK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHVGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWWEDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6861..6880)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6905..6953
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(8004..8022)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8043..8059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8043..8059)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8056..8078)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8067..8083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8091..8097,8098..8115,8116..8121))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8274..8291)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8445..9033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8525..8544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9204..9995,9996..10064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9827..9846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10065..10169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10237..10255
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac accttttcaa ttcatcattt tttttttatt cttttttttg atttcggttt
      241 ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
      301 agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
      361 ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa
      421 gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
      481 atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
      541 ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
      601 atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
      661 tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg
      721 cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
      781 gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
      841 cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
      901 tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
      961 gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
     1021 gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg
     1081 gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
     1141 gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
     1201 ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
     1261 agagcttcaa tttaattata tcagttatta ccctatgcgg tgtgaaatac cgcacagatg
     1321 cgtaaggaga aaataccgca tcaggaaatt gtaaacgtta atattttgtt aaaattcgcg
     1381 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1441 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1501 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1561 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     1621 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1681 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1741 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1801 tcgcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     1861 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     1921 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     1981 ctatagggcg aattggagct ccaccgcggt ggcggccgcc tcgagtcaca caattgcccg
     2041 gaacaggaag gagaaaacag cccccagtgc cagacccaga cacaggaaga aggccatgat
     2101 ggctcctgcg gtctctgcct cagctggctt cactttcttg ggcccgaagc acatgcagag
     2161 gctggcgagg tagccgttgg agaaggcaaa ggcagccatg aagaagatga accaggcatc
     2221 gtgctcgaag accacagtca ggtagcggcg gggcttaatg ttgcacagca gcagcagtgg
     2281 cacaaacacc agccgggcca gcaccaggct tggcagccag cggctgtcct tcccaggcca
     2341 catgaataca gctgtgaggc tccggcccaa ccagtcaaag atattgaaag tcaagaaaca
     2401 ggacacagga atgaagtaac gttcccaggt gctgctgcct gcgatgctgg acttgacctc
     2461 aacagtcacg gctggaaaca tcccaatggt gatagtgaag atgaagcaga cagagaaagc
     2521 caggactgag atatttttca ggatggcttt gatagagtgg ctttcattgg tgggctgaga
     2581 gttggagact gaaactccag attcctcttt gcctgctctt ggctcctctc ctttgctaat
     2641 gaggtccaac ttggtctcct gctccccggg tccttcaagc ttgagctgct ggtagtagcg
     2701 gtagaattcc aggcggggca ggcccaggta acagatgatg gtcaaaatga taacagcaca
     2761 ggctgtgata aagtagccga aggcactttc tgatagctcc gagccactgg caatagcgca
     2821 gatcatggcc acggaggcaa agaagcctgc taggccctgg ccactcatga tgggggccgt
     2881 gtagctggca ggcagaaggc cagccagacc aaacaggctg ccctgcagga tggcaccaaa
     2941 tgaattaatg agcacgatct tgatcatggt gatgacaaag aagggcagag catccagctg
     3001 caccttcacc aggatggcag tgatcagaaa caccagcagg atggccacca ggctgcccag
     3061 gatccgtacg gactggggga tcctctgatg caggaaggag ttgaggtagg tgaataacag
     3121 caggggcagc atggcacata gggtcatgac attgttgaag atggcactga gagagttccg
     3181 ctcaggcaag ggtgctgcag gggcggctga cgcctgggcg tccttgctca gttcagcagt
     3241 gaccaaggac acattctggg acatgtccag gcggtttgtg aaatactgag tggccgtcat
     3301 gaaaaaattc cacgggagca gcgttcccag acccagcatg aagaagataa gccagacagc
     3361 tttgtatctg tcctgaggct ggtgactggt tgtcatagat cccccgggct gcaggaattc
     3421 gatatcaagc ttatcgataa gcttgatatc gaattccgag cttggagttg attgtatgct
     3481 tggtatagct tgaaatattg tgcagaaaaa gaaacaagga agaaagggaa cgagaacaat
     3541 gacgaggaaa caaaagatta ataattgcag gtctatttat acttgatagc aagacagcaa
     3601 actttttttt atttcaaatt caagtaactg gaaggaaggc cgtataccgt tgctcattag
     3661 agagtagtgt gcgtgaatga aggaaggaaa aagtttcgtg tgcttcgaga tacccctcat
     3721 cagctctgga acaacgacat ctgttggtgc tgtctttgtc gttaattttt tcctttagtg
     3781 tcttccatca tttttttgtc attgcggata tggtgagaca acaacggggg agagagaaaa
     3841 gaaaaaaaaa gaaaagaagt tgcatgcgcc tattattact tcaatagatg gcaaatggaa
     3901 aaagggtagt gaaacttcga tatgatgatg gctatcaagt ctagggctac agtattagtt
     3961 cgttatgtac caccatcaat gaggcagtgt aattggtgta gtctttgttt agcccattat
     4021 gtcttgctgg gatctgttct atgatatctc ccctccgcca cctacatgtt agggagacca
     4081 ccgaaggtat tataggaatc cccgatgttg ggttttggtt gccagaaaag aggaagtcca
     4141 tattgtacac ccggaaacaa caaaaggata tccgaaatat tccacggttt agaaaaaaat
     4201 cggaaaagag cgcggagggg tgttaccccc cttctctact agcattggac tttaattaat
     4261 atatgtgctt agagaagtgt aaagttccct tccatattgt aacataataa agtgcacacc
     4321 caaatgaatt gaaagcgtac tcaaacagac aaccatttcc agtgttgtat gtacctgtct
     4381 atttatactg gtagcaaccc tattgctgtt tcctcttcaa agtactctag cggttatgcg
     4441 cgtctcacct tcaaggtcat ggtcgctcta ttgttcgcac caccggcaaa ctcgcgtctc
     4501 gcaagtcttg gctcattctt ctagtatact catgttgcaa atgcactcag gttctttcgg
     4561 caacttaaat aatgacacca gttgtcgtgg tcgtcatcat cgcaacccca accggcattc
     4621 ttattgcttc tccaatctcg ccccttagcg cagggtaaac cttggaaaat gcaggcgcaa
     4681 aaaactccgc cgggcacagc ctcacgccca gcgttatcgc cgggccggca agagcgcggg
     4741 tccgccacag agtcagcatg attgtgcaat tgcgtaaact cgttttttcg gcgccgcaaa
     4801 gccaaataca tcatatcaac acttttcact ttatttttcg ttcgaccctt atatttgtct
     4861 tttgccttca tgctccttga tttcctattt catttaccat catttcttcg atccccctca
     4921 tcgtgatgta gtcaaccctc ttcttgtttt aggatcctct agaactagtg gatcccccgg
     4981 gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcatgtttg acagcttatc
     5041 atcgataagc ttggctgcag gtcgagttta tcattatcaa tactgccatt tcaaagaata
     5101 cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat tagcctttta
     5161 attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa tatataacat
     5221 cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg gagcccgctt
     5281 tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca
     5341 accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg
     5401 caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca
     5461 aggcaattga cccacgcatg tatctatctc attttcttac accttctatt accttctgct
     5521 ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattattccc
     5581 ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt
     5641 tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa
     5701 gaacttagtt tcgacggatc cgcgaattca ccatggcttc gtacccctgc catcaacacg
     5761 cgtctgcgtt cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc
     5821 gccctcgccg gcagcaagaa gccacggaag tccgcctgga gcggaaaatg cccacgctac
     5881 tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg caactgctgg
     5941 tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcagg
     6001 tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg
     6061 gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca
     6121 tgccttatgc cgtgaccgac gccgttctgg ctcctcatgt cgggggggag gctgggagtt
     6181 cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc
     6241 tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc gtgctggcgt
     6301 tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg gcccttccgg
     6361 aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg cttgacctgg
     6421 ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg cggtatctgc
     6481 agggcggcgg gtcgtggtgg gaggattggg gacagctttc ggggacggcc gtgccgcccc
     6541 agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta
     6601 ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct
     6661 gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc ctggattacg
     6721 accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga
     6781 cccacgtcac caccccaggc tccataccga cgatctgcga cctggcgcgc acgtttgccc
     6841 gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     6901 atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg
     6961 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     7021 atacgcccgc gtttcttcct tttccccacc ccaaccccca agttcgggtg aaggcccagg
     7081 gctcgcagcc aacgtcgggg cggcaagccc tgccatagcc acgggccccg tgggttaggg
     7141 acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattctttt gggcgttgcg
     7201 tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga tggcctgggc
     7261 atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc aaacaccccc
     7321 gacccccaaa aaccaccgcg cggatttctg gcgccgccgg acgaactaaa cctgactacg
     7381 gcatctctgc cccttcttcg ctggtacgag gagcgctttt gttttgtatt ggtcaccacg
     7441 gccgagtttc cgcgggaccc cggccagctg ctttacatct cgaagaccta cctactcggc
     7501 cggcccccga acgcgagcct gcccgccccc atcacggtcg agccgaccgc ccagcctccc
     7561 cccgcggtcg ccccccttaa gggtctcttg cacaatccaa ccgcctccgt gttgctgcgt
     7621 tcccgggcct gggtaacgtt ttcggccgtc cctgaccccg aggccctgac gttcccgcgg
     7681 ggagacaacg tggcgacggc gagccacccg agcgggccgc gtgatacacc gcccccccga
     7741 ccgccggttg gggcccggcg gcacccgacg acggagctgg acatcacgca cctgcacaac
     7801 gcgtccacga cctggttggc cacccggggc ctgttgagat ccccaggtag gtacgtgtat
     7861 ttctccccgt cggcctcgac gtggcccgtg ggcatctgga cgacggggga gctggtgctc
     7921 gggtgcgatg ccgcgctggt gcgcgcgcgc tacgggcggg aattcgcgga tccactagat
     7981 ccccgggtac ccagcttttg ttccctttag tgagggttaa ttccgagctt ggcgtaatca
     8041 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8101 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
     8161 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8221 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8881 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10201 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10261 cgtc
//
