LOCUS       sequence-137615-       10418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BrdU-incorporating plasmid with selectable marker HIS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Viggiani CJ, Aparicio OM
  TITLE     New vectors for simplified construction of BrdU-Incorporating 
            strains of Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2006 Oct-Nov;23(14-15):1045-51.
  PUBMED    17083135
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2006
            Oct-Nov"; volume: "23(14-15)"; pages: "1045-51"
COMMENT     Sequence Label: p403-BrdU-Inc
FEATURES             Location/Qualifiers
     source          1..10418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        317..504
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             505..1167
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     rep_origin      complement(1427..1882)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1564..1585)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1776..1795
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2012..2034
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2026..2043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2027..2043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2053..2072
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2053..2071
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2096..2112
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2097..2113
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2147..2163)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2147..2163)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3618..4321)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        5172..5815
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5708..5731
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             5834..6964
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLERK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHVGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWWEDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(6962..6981)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7006..7054
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(8158..8178)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8158..8176)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8197..8213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8197..8213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8210..8232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8221..8237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8245..8251,8252..8269,8270..8275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8428..8445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8599..9187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8679..8698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9358..10149,10150..10218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9981..10000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10219..10323)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10391..10409
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt ccacctagcg gatgactctt tttttttctt agcgattggc attatcacat
      421 aatgaattat acattatata aagtaatgtg atttcttcga agaatatact aaaaaatgag
      481 caggcaagat aaacgaaggc aaagatgaca gagcagaaag ccctagtaaa gcgtattaca
      541 aatgaaacca agattcagat tgcgatctct ttaaagggtg gtcccctagc gatagagcac
      601 tcgatcttcc cagaaaaaga ggcagaagca gtagcagaac aggccacaca atcgcaagtg
      661 attaacgtcc acacaggtat agggtttctg gaccatatga tacatgctct ggccaagcat
      721 tccggctggt cgctaatcgt tgagtgcatt ggtgacttac acatagacga ccatcacacc
      781 actgaagact gcgggattgc tctcggtcaa gcttttaaag aggccctagg ggccgtgcgt
      841 ggagtaaaaa ggtttggatc aggatttgcg cctttggatg aggcactttc cagagcggtg
      901 gtagatcttt cgaacaggcc gtacgcagtt gtcgaacttg gtttgcaaag ggagaaagta
      961 ggagatctct cttgcgagat gatcccgcat tttcttgaaa gctttgcaga ggctagcaga
     1021 attaccctcc acgttgattg tctgcgaggc aagaatgatc atcaccgtag tgagagtgcg
     1081 ttcaaggctc ttgcggttgc cataagagaa gccacctcgc ccaatggtac caacgatgtt
     1141 ccctccacca aaggtgttct tatgtagtga caccgattat ttaaagctgc agcatacgat
     1201 atatatacat gtgtatatat gtatacctat gaatgtcagt aagtatgtat acgaacagta
     1261 tgatactgaa gatgacaagg taatgcatca ttctatacgt gtcattctga acgaggcgcg
     1321 ctttcctttt ttctttttgc tttttctttt tttttctctt gaactcgacg gatctatgcg
     1381 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt
     1441 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     1501 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     1561 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     1621 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     1681 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     1741 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     1801 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     1861 aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa
     1921 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     1981 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     2041 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
     2101 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
     2161 agagcggccg cctcgagtca cacaattgcc cggaacagga aggagaaaac agcccccagt
     2221 gccagaccca gacacaggaa gaaggccatg atggctcctg cggtctctgc ctcagctggc
     2281 ttcactttct tgggcccgaa gcacatgcag aggctggcga ggtagccgtt ggagaaggca
     2341 aaggcagcca tgaagaagat gaaccaggca tcgtgctcga agaccacagt caggtagcgg
     2401 cggggcttaa tgttgcacag cagcagcagt ggcacaaaca ccagccgggc cagcaccagg
     2461 cttggcagcc agcggctgtc cttcccaggc cacatgaata cagctgtgag gctccggccc
     2521 aaccagtcaa agatattgaa agtcaagaaa caggacacag gaatgaagta acgttcccag
     2581 gtgctgctgc ctgcgatgct ggacttgacc tcaacagtca cggctggaaa catcccaatg
     2641 gtgatagtga agatgaagca gacagagaaa gccaggactg agatattttt caggatggct
     2701 ttgatagagt ggctttcatt ggtgggctga gagttggaga ctgaaactcc agattcctct
     2761 ttgcctgctc ttggctcctc tcctttgcta atgaggtcca acttggtctc ctgctccccg
     2821 ggtccttcaa gcttgagctg ctggtagtag cggtagaatt ccaggcgggg caggcccagg
     2881 taacagatga tggtcaaaat gataacagca caggctgtga taaagtagcc gaaggcactt
     2941 tctgatagct ccgagccact ggcaatagcg cagatcatgg ccacggaggc aaagaagcct
     3001 gctaggccct ggccactcat gatgggggcc gtgtagctgg caggcagaag gccagccaga
     3061 ccaaacaggc tgccctgcag gatggcacca aatgaattaa tgagcacgat cttgatcatg
     3121 gtgatgacaa agaagggcag agcatccagc tgcaccttca ccaggatggc agtgatcaga
     3181 aacaccagca ggatggccac caggctgccc aggatccgta cggactgggg gatcctctga
     3241 tgcaggaagg agttgaggta ggtgaataac agcaggggca gcatggcaca tagggtcatg
     3301 acattgttga agatggcact gagagagttc cgctcaggca agggtgctgc aggggcggct
     3361 gacgcctggg cgtccttgct cagttcagca gtgaccaagg acacattctg ggacatgtcc
     3421 aggcggtttg tgaaatactg agtggccgtc atgaaaaaat tccacgggag cagcgttccc
     3481 agacccagca tgaagaagat aagccagaca gctttgtatc tgtcctgagg ctggtgactg
     3541 gttgtcatag atcccccggg ctgcaggaat tcgatatcaa gcttatcgat aagcttgata
     3601 tcgaattccg agcttggagt tgattgtatg cttggtatag cttgaaatat tgtgcagaaa
     3661 aagaaacaag gaagaaaggg aacgagaaca atgacgagga aacaaaagat taataattgc
     3721 aggtctattt atacttgata gcaagacagc aaactttttt ttatttcaaa ttcaagtaac
     3781 tggaaggaag gccgtatacc gttgctcatt agagagtagt gtgcgtgaat gaaggaagga
     3841 aaaagtttcg tgtgcttcga gatacccctc atcagctctg gaacaacgac atctgttggt
     3901 gctgtctttg tcgttaattt tttcctttag tgtcttccat catttttttg tcattgcgga
     3961 tatggtgaga caacaacggg ggagagagaa aagaaaaaaa aagaaaagaa gttgcatgcg
     4021 cctattatta cttcaataga tggcaaatgg aaaaagggta gtgaaacttc gatatgatga
     4081 tggctatcaa gtctagggct acagtattag ttcgttatgt accaccatca atgaggcagt
     4141 gtaattggtg tagtctttgt ttagcccatt atgtcttgct gggatctgtt ctatgatatc
     4201 tcccctccgc cacctacatg ttagggagac caccgaaggt attataggaa tccccgatgt
     4261 tgggttttgg ttgccagaaa agaggaagtc catattgtac acccggaaac aacaaaagga
     4321 tatccgaaat attccacggt ttagaaaaaa atcggaaaag agcgcggagg ggtgttaccc
     4381 cccttctcta ctagcattgg actttaatta atatatgtgc ttagagaagt gtaaagttcc
     4441 cttccatatt gtaacataat aaagtgcaca cccaaatgaa ttgaaagcgt actcaaacag
     4501 acaaccattt ccagtgttgt atgtacctgt ctatttatac tggtagcaac cctattgctg
     4561 tttcctcttc aaagtactct agcggttatg cgcgtctcac cttcaaggtc atggtcgctc
     4621 tattgttcgc accaccggca aactcgcgtc tcgcaagtct tggctcattc ttctagtata
     4681 ctcatgttgc aaatgcactc aggttctttc ggcaacttaa ataatgacac cagttgtcgt
     4741 ggtcgtcatc atcgcaaccc caaccggcat tcttattgct tctccaatct cgccccttag
     4801 cgcagggtaa accttggaaa atgcaggcgc aaaaaactcc gccgggcaca gcctcacgcc
     4861 cagcgttatc gccgggccgg caagagcgcg ggtccgccac agagtcagca tgattgtgca
     4921 attgcgtaaa ctcgtttttt cggcgccgca aagccaaata catcatatca acacttttca
     4981 ctttattttt cgttcgaccc ttatatttgt cttttgcctt catgctcctt gatttcctat
     5041 ttcatttacc atcatttctt cgatccccct catcgtgatg tagtcaaccc tcttcttgtt
     5101 ttaggatcct ctagagtcga cctcatgttt gacagcttat catcgataag cttggctgca
     5161 ggtcgagttt atcattatca atactgccat ttcaaagaat acgtaaataa ttaatagtag
     5221 tgattttcct aactttattt agtcaaaaaa ttagcctttt aattctgctg taacccgtac
     5281 atgcccaaaa tagggggcgg gttacacaga atatataaca tcgtaggtgt ctgggtgaac
     5341 agtttattcc tggcatccac taaatataat ggagcccgct ttttaagctg gcatccagaa
     5401 aaaaaaagaa tcccagcacc aaaatattgt tttcttcacc aaccatcagt tcataggtcc
     5461 attctcttag cgcaactaca gagaacaggg gcacaaacag gcaaaaaacg ggcacaacct
     5521 caatggagtg atgcaacctg cctggagtaa atgatgacac aaggcaattg acccacgcat
     5581 gtatctatct cattttctta caccttctat taccttctgc tctctctgat ttggaaaaag
     5641 ctgaaaaaaa aggttgaaac cagttccctg aaattattcc cctacttgac taataagtat
     5701 ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact tcttaaattc
     5761 tacttttata gttagtcttt tttttagttt taaaacacca agaacttagt ttcgacggat
     5821 ccgcgaattc accatggctt cgtacccctg ccatcaacac gcgtctgcgt tcgaccaggc
     5881 tgcgcgttct cgcggccata gcaaccgacg tacggcgttg cgccctcgcc ggcagcaaga
     5941 agccacggaa gtccgcctgg agcggaaaat gcccacgcta ctgcgggttt atatagacgg
     6001 tcctcacggg atggggaaaa ccaccaccac gcaactgctg gtggccctgg gttcgcgcga
     6061 cgatatcgtc tacgtacccg agccgatgac ttactggcag gtgctggggg cttccgagac
     6121 aatcgcgaac atctacacca cacaacaccg cctcgaccag ggtgagatat cggccgggga
     6181 cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc atgccttatg ccgtgaccga
     6241 cgccgttctg gctcctcatg tcggggggga ggctgggagt tcacatgccc cgcccccggc
     6301 cctcaccctc atcttcgacc gccatcccat cgccgccctc ctgtgctacc cggccgcgcg
     6361 ataccttatg ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc
     6421 gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg gaggacagac acatcgaccg
     6481 cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg gctatgctgg ccgcgattcg
     6541 ccgcgtttac gggctgcttg ccaatacggt gcggtatctg cagggcggcg ggtcgtggtg
     6601 ggaggattgg ggacagcttt cggggacggc cgtgccgccc cagggtgccg agccccagag
     6661 caacgcgggc ccacgacccc atatcgggga cacgttattt accctgtttc gggcccccga
     6721 gttgctggcc cccaacggcg acctgtataa cgtgtttgcc tgggccttgg acgtcttggc
     6781 caaacgcctc cgtcccatgc acgtctttat cctggattac gaccaatcgc ccgccggctg
     6841 ccgggacgcc ctgctgcaac ttacctccgg gatggtccag acccacgtca ccaccccagg
     6901 ctccataccg acgatctgcg acctggcgcg cacgtttgcc cgggagatgg gggaggctaa
     6961 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     7021 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     7081 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     7141 ttttccccac cccaaccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     7201 gcggcaagcc ctgccatagc cacgggcccc gtgggttagg gacggggtcc cccatgggga
     7261 atggtttatg gttcgtgggg gttattcttt tgggcgttgc gtggggtcag gtccacgact
     7321 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
     7381 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
     7441 gcggatttct ggcgccgccg gacgaactaa acctgactac ggcatctctg ccccttcttc
     7501 gctggtacga ggagcgcttt tgttttgtat tggtcaccac ggccgagttt ccgcgggacc
     7561 ccggccagct gctttacatc tcgaagacct acctactcgg ccggcccccg aacgcgagcc
     7621 tgcccgcccc catcacggtc gagccgaccg cccagcctcc ccccgcggtc gcccccctta
     7681 agggtctctt gcacaatcca accgcctccg tgttgctgcg ttcccgggcc tgggtaacgt
     7741 tttcggccgt ccctgacccc gaggccctga cgttcccgcg gggagacaac gtggcgacgg
     7801 cgagccaccc gagcgggccg cgtgatacac cgcccccccg accgccggtt ggggcccggc
     7861 ggcacccgac gacggagctg gacatcacgc acctgcacaa cgcgtccacg acctggttgg
     7921 ccacccgggg cctgttgaga tccccaggta ggtacgtgta tttctccccg tcggcctcga
     7981 cgtggcccgt gggcatctgg acgacggggg agctggtgct cgggtgcgat gccgcgctgg
     8041 tgcgcgcgcg ctacgggcgg gaattcgcgg atccactaga tccccgggta cccaatttcg
     8101 ccctataagt gagttcgtat tacgcgcgtt cactggcttg agctccagct tttgttccct
     8161 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8221 ttgttatccg ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg
     8281 gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8341 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8401 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8461 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8521 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8581 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8641 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8701 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8761 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8821 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8881 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8941 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9001 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9061 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9121 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9181 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9241 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9301 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9361 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9421 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9481 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9541 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9601 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9661 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9721 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9781 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9841 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9901 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9961 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10021 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10081 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10141 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10201 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10261 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10321 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10381 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
