LOCUS       sequence-137617-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused
            proteins with C-terminal 6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-3
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(213..239)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(258..281)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(309..326)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa
       61 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
      121 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
      181 tgaattatcg cgatactagt tctagattat caggcgtagt cgggcacgtc gtagggatat
      241 ccttgatcat cttatcactt ctcgaactgg gggtggctcc acccggggtt aattaaacgc
      301 gtttatcaat ggtgatggtg atggtgctcg aggctagccg agctcgcggc ggccgcatta
      361 gtctgcgcgg ctgccagggc tgcatcgaca gtctgacgac cgctggcggc gttaatcacc
      421 gcagtgcgca cggcatacca gaaagcggac atctgcggga tgttgggcat gatttcacct
      481 ttctgggcgt tttccattgt ggcggcaaca cgtggatctt tcaccaactc ttcctcgtaa
      541 gacttcagcg ctacggcacc cagcggtttg tctttattaa ccgcttccag accttcatca
      601 gtcagcaggt agttttcgag gaactctttt gccagctctt tgttcggact ggcggcgtta
      661 atacctgcgc tcagcacgcc aacgaacggt ttggatggtt gacccttgaa ggtcggcagc
      721 accgttacac cataattcac tgcgctggtg tcgatgttgg accatgccca cgggccgttg
      781 atggtcatcg ctgtttcgcc atgattaaag gcatgttctg cgatggagta atcggtgtct
      841 gcattcatgt gtttgttttt aatcaggtca accaggaagg tcagacccgc tttcgcgcca
      901 gcgttatcca cgcccacgtc tttaatgtcg tactttccgg ctgcatactt gaacgcataa
      961 cccccgtcag cagcaatcag cggccaggtg aagtacggtt cttgcaggtt gaacatcagc
     1021 gcgctcttac ctttcgcttt cagttcttta tccagcgccg ggatctcttc ccaggtttt
//
