LOCUS       sequence-137620-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused
            proteins with C-terminal 6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-2
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagaattgcg gccgtctcag gccgaattcg tcgacaagct tgccaccatg gggatccttc
       61 ccagccctgg gatgcctgcg ctgctctccc tcgtgagcct tctctccgtg ctgctgatgg
      121 gttgcgtagc tgaaaccggt caccatcacc atcaccataa aaccgaagaa ggtaaactgg
      181 taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg
      241 agaaagatac cggaattaaa gtcaccgttg agcatcccga taaactggaa gagaaattcc
      301 cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg
      361 gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggccgcagcg ttccaggaca
      421 agctgtatcc gtttacctgg gatgccgtgc gctacaacgg caagctgatt gcttacccga
      481 tcgctgttga agcgttgtcc ctgatttata acaaggatct gctgccgaac ccgccaaaaa
      541 cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga
      601 tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg
      661 cgttcaagta tgcagccgga aagtacgaca ttaaagacgt gggcgtggat aacgctggcg
      721 cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca
      781 ccgattactc catcgcagaa catgccttta atcatggcga aacagcgatg accatcaacg
      841 gcccgtgggc atggtccaac atcgacacca gcgcagtgaa ttatggtgta acggtgctgc
      901 cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg
      961 ccgccagtcc gaacaaagag ctggcaaaa
//
