LOCUS       sequence-137627-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked 
            fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-8
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaattgcg gccgtctcag gccgaattcg ccaccatgaa aactgaagaa ggtaaactgg
       61 taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg
      121 agaaagatac cggaattaaa gtcaccgttg agcatcccga taaactggaa gagaaattcc
      181 cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg
      241 gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggccgcagcg ttccaggaca
      301 agctgtatcc gtttacctgg gatgccgtgc gctacaacgg caagctgatt gcttacccga
      361 tcgctgttga agcgttgtcc ctgatttata acaaggatct gctgccgaac ccgccaaaaa
      421 cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga
      481 tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg
      541 cgttcaagta tgcagccgga aagtacgaca ttaaagacgt gggcgtggat aacgctggcg
      601 cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg aatgcagaca
      661 ccgattactc catcgcagaa catgccttta atcatggcga aacagcgatg accatcaacg
      721 gcccgtgggc atggtccaac atcgacacca gcgcagtgaa ttatggtgta acggtgctgc
      781 cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg
      841 ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactacctg ctgactgatg
      901 aaggtctgga agcggttaat aaagacaaa
//
