LOCUS       sequence-137628-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked 
            fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-8
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(213..239)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(258..281)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(309..326)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(357..377)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa
       61 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
      121 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
      181 tgaattatcg cgatactagt tctagattat caggcgtagt cgggcacgtc gtagggatat
      241 ccttgatcat cttatcactt ctcgaactgg gggtggctcc acccggggtt aattaaacgc
      301 gtttatcaat ggtgatggtg atggtgctcg aggctagccg agctcgcggc ggccgcaccc
      361 tgaaaataaa gattctcgct ccctccgccg ctccctccgc cattagtctg cgcggctgcc
      421 agggctgcat cgacagtctg acgaccgctg gcggcgttaa tcaccgcagt gcgcacggca
      481 taccagaaag cggacatctg cgggatgttg ggcatgattt cacctttctg ggcgttttcc
      541 attgtggcgg caacacgtgg atctttcacc aactcttcct cgtaagactt cagcgctacg
      601 gcacccagcg gtttgtcttt attaaccgct tccagacctt catcagtcag caggtagttt
      661 tcgaggaact cttttgccag ctctttgttc ggactggcgg cgttaatacc tgcgctcagc
      721 acgccaacga acggtttgga tggttgaccc ttgaaggtcg gcagcaccgt tacaccataa
      781 ttcactgcgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt
      841 tcgccatgat taaaggcatg ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg
      901 tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc
      961 acgtctttaa tgtcgtactt tccggctgc
//
