LOCUS       sequence-137630-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV 
            cleavage site-linked fusion proteins with C-terminal 
            6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-10
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             141..164
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aagaattgcg gccgtctcag gccgaattcg tcgacaagct tgccaccatg gggatccttc
       61 ccagccctgg gatgcctgcg ctgctctccc tcgtgagcct tctctccgtg ctgctgatgg
      121 gttgcgtagc tgaaaccggt caccatcacc atcaccatca ccataaaacc gaagaaggta
      181 aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga
      241 aattcgagaa agataccgga attaaagtca ccgttgagca tcccgataaa ctggaagaga
      301 aattcccaca ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc
      361 gctttggtgg ctacgctcaa tctggcctgt tggctgaaat caccccggcc gcagcgttcc
      421 aggacaagct gtatccgttt acctgggatg ccgtgcgcta caacggcaag ctgattgctt
      481 acccgatcgc tgttgaagcg ttgtccctga tttataacaa ggatctgctg ccgaacccgc
      541 caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg
      601 cgctgatgtt caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg
      661 gttatgcgtt caagtatgca gccggaaagt acgacattaa agacgtgggc gtggataacg
      721 ctggcgcgaa agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg
      781 cagacaccga ttactccatc gcagaacatg cctttaatca tggcgaaaca gcgatgacca
      841 tcaacggccc gtgggcatgg tccaacatcg acaccagcgc agtgaattat ggtgtaacgg
      901 tgctgccgac cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta
      961 ttaacgccgc cagtccgaa
//
