LOCUS       sequence-137636-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human Fibrillarin with C-terminal 3xFLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Stamm
  TITLE     p3xFLAG_Fibrillarin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV14_Fibrillarin
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(180..245)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             441..452
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 cttgcccctt gctccatacc acccccctcc accccataat attatagaag gacacctagt
       61 cagacaaaat gatgcaactt aattttatta ggacaaggct ggtgggcact ggagtggcaa
      121 cttccagggc caggagaggc actggggagg ggtcacaggg atgccacccg ggatcactac
      181 ttgtcatcgt catccttgta gtcgatgtca tgatctttat aatcaccgtc atggtctttg
      241 tagtcagccc gggatcctct agagttcttc accttggggg gtggcctgta cactcccacg
      301 accacggcat ggtctctttc atatggctca agggtcaact gctcctgcgg cttcatgttc
      361 tcctgttgca tctttttcac ttcggaggca aacacggcct cggctgaggc tgtggagtca
      421 atgcagttgg ccttaatgga aatcacaaag tgtcctccat tacgcaggaa ggtgtgggca
      481 ttcagggcca caatccgggt ctggtctggc tgggccacat cagcaaagat cacatccacc
      541 attgcgatga gcatgcggta tttgtgtggg tgtcgagcat cctcgatcac aggaatgatg
      601 ttggtcctct tcttggccaa gttaatgagg tcacggccag agcggtggga gaactcgact
      661 gcatagacta gaccatccgg accaacgatg tcagagacat gggagaccgt ggtgcccgag
      721 gcagccccga ggtagagaac cttagccccc ggtttgatgt ggatctggtc cacaccaccc
      781 aggattgctg ctgctagctt gg
//
