LOCUS       sequence-137642-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human SNORD27 (C/D box snoRNA U27).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD27h
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             521..562
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             572..589
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(612..629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    618..729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattg
      121 cccttatgtt cagcccacaa gagcttactg gtgaaggaat gggacaagac ccatctttat
      181 gcaaagccag cgttacagta atgttccagg taggtgtaca tggtttatgc tcttacagag
      241 gagaccttgt agataaccac tccatgatga acacaaaatg acaagcatat ggctgaactt
      301 tcaagtgatg tcatcttact actgagaagt gagagagagg tcttaagggg tctttgaatg
      361 actattttta ggtacataaa atgctttcct ctgttgtcta cagcatctca taatctatcc
      421 tggggaattc agctgcctcc cagggtgaat acagaagggc aattctgcag atatccagca
      481 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
      541 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
      601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      781 cagcaa
//
