LOCUS       sequence-137643-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human SNORD27 (C/D box snoRNA U27).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD27h
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..63)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(530..549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(531..549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(594..797)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(623..643)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgatga tgaccggtac gcgtagaatc gagaccgagg agagggttag ggataggctt
       61 accttcgaac cgcgggccct ctagactcga gcggccgcca ctgtgctgga tatctgcaga
      121 attgcccttc tgtattcacc ctgggaggca gctgaattcc ccaggataga ttatgagatg
      181 ctgtagacaa cagaggaaag cattttatgt acctaaaaat agtcattcaa agacccctta
      241 agacctctct ctcacttctc agtagtaaga tgacatcact tgaaagttca gccatatgct
      301 tgtcattttg tgttcatcat ggagtggtta tctacaaggt ctcctctgta agagcataaa
      361 ccatgtacac ctacctggaa cattactgta acgctggctt tgcataaaga tgggtcttgt
      421 cccattcctt caccagtaag ctcttgtggg ctgaacataa gggcaattcc accacactgg
      481 actagtggat ccgagctcgg taccaagctt aactagccag cttgggtctc cctatagtga
      541 gtcgtattaa tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg
      601 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
      661 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      721 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
      781 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
      841 g
//
