LOCUS       sequence-137648-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human mutated SNORD27 (C/D box snoRNA U27).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD27h_115mut
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             530..571
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             581..598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(621..638)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    627..738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctagagaacc cactgcctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 gcccttatgt tcagcccaca agagcttact ggtgaaggaa tgggacaaga cccatcttta
      181 tgcaaagcca gcgttacagt aatgttccag gtaggtgtac atggtttatg ctcttacaga
      241 ggagaccttg tagataacca ctccatgatg aacacaaaat gacaagcata tggctgaact
      301 ttcaagtgat gtatgctcaa taggattacg ctgagaagtg agagagaggt cttaaggggt
      361 ctttgaatga ctatttttag gtacataaaa tgctttcctc tgttgtctac agcatctcat
      421 aatctatcct ggggaattca gctgcctccc agggtgaata cagaagggca attctgcaga
      481 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
      541 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
      601 agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      661 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      721 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
      781 ggggcaggac agcaaggg
//
