LOCUS       sequence-137649-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression clone for human mutated SNORD27 (C/D box snoRNA U27).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pcDNA3.1_SNORD27h_115mut
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(536..555)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(537..555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(600..699)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(629..649)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaccg cgggccctct agactcgagc ggccgccact gtgctggata tctgcagaat
      121 tgcccttctg tattcaccct gggaggcagc tgaattcccc aggatagatt atgagatgct
      181 gtagacaaca gaggaaagca ttttatgtac ctaaaaatag tcattcaaag accccttaag
      241 acctctctct cacttctcag cgtaatccta ttgagcatac atcacttgaa agttcagcca
      301 tatgcttgtc attttgtgtt catcatggag tggttatcta caaggtctcc tctgtaagag
      361 cataaaccat gtacacctac ctggaacatt actgtaacgc tggctttgca taaagatggg
      421 tcttgtccca ttccttcacc agtaagctct tgtgggctga acataagggc aattccacca
      481 cactggacta gtggatccga gctcggtacc aagcttaact agccagcttg ggtctcccta
      541 tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga
      601 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
      661 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
      721 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
      781 atgtactgcc aa
//
