LOCUS       sequence-137652-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  antisense probe for detection of human SNORD27.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov
            K, Nevo Y, Eyras E, Sperling R, Stamm S
  TITLE     Dual function of C/D box small nucleolar RNAs in rRNA modification 
            and alternative pre-mRNA splicing.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 
            10.1073/pnas.1519292113. Epub 2016 Mar 8.
  PUBMED    26957605
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1519292113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-03-22- 22"; volume: "113"; issue: "12"; pages: 
            "E1625-34"
COMMENT     Sequence Label: pCR4_SNORD27 probe
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(154..173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(155..173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(180..197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(180..196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(189..211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             334..636
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     515..534
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaagggacta gtcctgcagg tttaaacgaa ttcgccctta accactccat gatgaacaca
       61 aaatgacaag catatggctg aactttcaag tgatgtcatc ttactactga gaagtgagag
      121 aagggcgaat tcgcggccgc taaattcaat tcgccctata gtgagtcgta ttacaattca
      181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      301 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga
      361 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga
      421 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
      481 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
      541 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
      601 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa
      661 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc
      721 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
      781 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
      841 agcgaaccgg aattgccagc tgg
//
