LOCUS       sequence-137659-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV 
            cleavage site-linked fusion proteins with C-terminal 
            6His-tag/Strep-tag II/HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Bokhove M, Sadat Al Hosseini H, Saito T, Dioguardi E, 
            Gegenschatz-Schmid K, Nishimura K, Raj I, de Sanctis D, Han L, 
            Jovine L
  TITLE     Easy mammalian expression and crystallography of maltose-binding 
            protein-fused human proteins.
  JOURNAL   J Struct Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi:
            10.1016/j.jsb.2016.01.016.
  PUBMED    26850170
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Struct 
            Biol. 2016 Feb 3. 194:1-7. pii: S1047-8477(16)30015-6. doi: 
            10.1016/j.jsb.2016.01.016."
COMMENT     Sequence Label: pHLmMBP-10
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(213..239)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(258..281)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(309..326)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(357..377)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa
       61 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
      121 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
      181 tgaattatcg cgatactagt tctagattat caggcgtagt cgggcacgtc gtagggatat
      241 ccttgatcat cttatcactt ctcgaactgg gggtggctcc acccggggtt aattaaacgc
      301 gtttatcaat ggtgatggtg atggtgctcg aggctagccg agctcgcggc ggccgcgctc
      361 tgaaaataca ggttttcgct ccctccgccg ctccctccgc ccttggtgat attagtctgc
      421 gcggctgcca gggctgcatc gacagtctga cgaccgctgg cggcgttaat caccgcagtg
      481 cgcacggcat accagaaagc ggacatctgc gggatgttgg gcatgatttc acctttctgg
      541 gcgttttcca ttgtggcggc aacacgtgga tctttcacca actcttcctc gtaagacttc
      601 agcgctacgg cacccagcgg tttgtcttta ttaaccgctt ccagaccttc atcagtcagc
      661 aggtagtttt cgaggaactc ttttgccagc tctttgttcg gactggcggc gttaatacct
      721 gcgctcagca cgccaacgaa cggtttggat ggttgaccct tgaaggtcgg cagcaccgtt
      781 acaccataat tcactgcgct ggtgtcgatg ttggaccatg cccacgggcc gttgatggtc
      841 atcgctgttt cgccatgatt aaaggcatgt tctgcgatgg agtaatcggt gtctgcattc
      901 atgtgtttg
//
