LOCUS       sequence-137674-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible sucrose counter-selection allelic exchange vector
            for Pseudomonas syringae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Mijatovic J, Severns PM, Kemerait RC, Walcott RR, Kvitko BH
  TITLE     Patterns of Seed-to-Seedling Transmission of Xanthomonas citri pv. 
            malvacearum, the Causal Agent of Cotton Bacterial Blight.
  JOURNAL   Phytopathology. 2021 Dec;111(12):2176-2184. doi: 
            10.1094/PHYTO-02-21-0057-R. Epub 2021 Nov 22.
  PUBMED    34032522
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/PHYTO-02-21-0057-R"; journalName: "Phytopathology"; date: 
            "2021-12"; volume: "111"; issue: "12"; pages: "2176-2184"
COMMENT     Sequence Label: pDEST1K18ms
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     517..539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     531..548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     532..548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    605..729
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
       61 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      121 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
      181 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
      241 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
      301 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
      361 gtgcttgcgg cagcgtgaag ctagcttatc gccattcgcc attcaggctg cgcaactgtt
      421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
      481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
      541 cggccagtgc caagcttgca tgcctgcagg tcgactctag aggatccccc tcgggtatgc
      601 atgcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
      661 attaaattag attttgcata aaaaacagac tacataatac tgtaagacac aacatatcca
      721 gtcactatgg tcgacctgca
//
