LOCUS       sequence-137689-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo protein protein interaction studies/ expression in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  TITLE     pGolt1-mCherry, expression in mammalian cell
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGolt1-mCherry
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             214..924
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(359..377)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     627..646
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tatgagcctc
      121 atctgcgcct tcgccatcgc cttcatcatc agtgtcatgc tcatcgcggc caaccaggtg
      181 ctgcgcagtg gcatgaagcc accggtcgcc accatggtga gcaagggcga ggaggataac
      241 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      301 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      361 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      421 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      481 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      541 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      601 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      661 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      721 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      781 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      841 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      901 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca
      961 catttgtaga ggttt
//
