LOCUS       sequence-137690-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo protein protein interaction studies/ expression in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  TITLE     pGolt1-mCherry, expression in mammalian cell
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGolt1-mCherry
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..108)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      135..590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(222..241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     432..453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    597..718
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(608..627)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     662..681
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aggactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
       61 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      121 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      181 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      241 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      301 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      361 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      421 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      481 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      541 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt acgccttaag
      601 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      661 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      721 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      781 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      841 ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gttcgtactg
      901 ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt tgacgttgta
      961 ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta
//
