LOCUS       sequence-137692-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo protein protein interaction studies/ expression in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  TITLE     pGolt2-mCherry, expression in mammalian cell
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGolt2-mCherry
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(9..113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      140..595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(227..246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     437..458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    602..723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(613..632)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     667..686
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
       61 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      121 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
      301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
      361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
      421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
      481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
      541 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttacgcc
      601 ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      661 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      721 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      781 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct agagtcgcgg
      841 ccgctacttc atgccactgc gcagcacctg gttggccgcg atgagcatga cactgatgat
      901 gaaggcgatg gcgaaggcgc agatgaggct tccggacttg tacagctcgt ccatgccgcc
      961 ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg a
//
