LOCUS       sequence-137693-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Visualization of Dendritic Golgi Satellite.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Mikhaylova M, Bera S, Kobler O, Frischknecht R, Kreutz MR
  TITLE     A Dendritic Golgi Satellite between ERGIC and Retromer.
  JOURNAL   Cell Rep. 2015 Dec 30. pii: S2211-1247(15)01437-0. doi: 
            10.1016/j.celrep.2015.12.024.
  PUBMED    26748700
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Dec 30. pii: S2211-1247(15)01437-0. doi: 
            10.1016/j.celrep.2015.12.024."
COMMENT     Sequence Label: pGolt-mCherry / pGolt3-mCherry
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    39..119
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             127..834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(272..290)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     540..559
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc
      121 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      181 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      241 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      301 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      361 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      421 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      481 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      541 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
      601 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
      661 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
      721 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
      781 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga
      841 ctggacgcac tagagatgcg gcgcattgac atttcccccg gactggacgc actagagatg
      901 cggcgcattg acatttcccc cggaagcctc atctgcgcct tcgccatcgc cttcatcatc
      961 agtgtcatgc tcatcgcggc caaccaggtg ctgcgcagtg gcatgaagta
//
