LOCUS       sequence-137701-        8384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB 
            protein plus the NPPS and CmR cassete in Synechocystis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Formighieri C, Melis A
  TITLE     Cyanobacterial production of plant essential oils.
  JOURNAL   Planta. 2018 Oct;248(4):933-946. doi: 10.1007/s00425-018-2948-0. 
            Epub 2018 Jul 4.
  PUBMED    29974209
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00425-018-2948-0"; journalName: "Planta"; date: "2018-10";
            volume: "248"; issue: "4"; pages: "933-946"
COMMENT     Sequence Label: CpcB-Solanum-lycopersicumPHLS_CpcA
FEATURES             Location/Qualifiers
     source          1..8384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(143..164)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     355..374
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4775..4877
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             4878..5537
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4944..4963)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6199..6217)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6238..6254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6238..6254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6251..6273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6262..6278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6286..6292,6293..6310,6311..6316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6331..6352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6469..6486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6640..7228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6720..6739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7399..8190,8191..8259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8022..8041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8260..8364)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagaagagtc cctgaatatc aaaatggtgg gataaaaagc tcaaaaagga
      721 aagtaggctg tggttcccta ggcaacagtc ttccctaccc cactggaaac taaaaaaacg
      781 agaaaagttc gcaccgaaca tcaattgcat aattttagcc ctaaaacata agctgaacga
      841 aactggttgt cttcccttcc caatccagga caatctgaga atcccctgca acattactta
      901 acaaaaaagc aggaataaaa ttaacaagat gtaacagaca taagtcccat caccgttgta
      961 taaagttaac tgtgggattg caaaagcatt caagcctagg cgctgagctg tttgagcatc
     1021 ccggtggccc ttgtcgctgc ctccgtgttt ctccctggat ttatttaggt aatatctctc
     1081 ataaatcccc gggtagttaa cgaaagttaa tggagatcag taacaataac tctagggtca
     1141 ttactttgga ctccctcagt ttatccgggg gaattgtgtt taagaaaatc ccaactcata
     1201 aagtcaagta ggagattaat tcaatgttcg acgtattcac tcgggttgtt tcccaagctg
     1261 atgctcgcgg cgagtacctc tctggttctc agttagatgc tttgagcgct accgttgctg
     1321 aaggcaacaa acggattgat tctgttaacc gcatcaccgg taatgcttcc gctatcgttt
     1381 ccaacgctgc tcgtgctttg ttcgccgaac agccccaatt aatccaaccc ggtggaaacg
     1441 cctacaccag ccgtcgtatg gctgcttgtt tgcgtgacat ggaaatcatc ctccgctatg
     1501 ttacctacgc aaccttcacc ggcgacgctt ccgttctaga agatcgttgc ttgaacggtc
     1561 tccgtgaaac ctacgttgcc ctgggtgttc ccggtgcttc cgtagctgct ggcgttcaaa
     1621 aaatgaaaga agctgccctg gacatcgtta acgatcccaa tggcatcacc cgtggtgatt
     1681 gcagtgctat cgttgctgaa atcgctggtt acttcgaccg cgccgctgct gccgtagccc
     1741 atatgtgctc ccattctacc acctcgtcca tgaatggttt tgaagatgcc cgggatcgaa
     1801 tccgtgaaag tttcggaaaa ctggaattgt ctcccagtag ctatgatacc gcgtgggtag
     1861 ccatggttcc aagccgtcat tctctgaatg aaccctgctt cccacagtgc ctggattgga
     1921 ttattgaaaa tcagcgggaa gatggctctt ggggtctaaa ccccacccat ccccttctgc
     1981 ttaaagactc cttatcctca accttagctt gtttattggc actcacaaag tggcgtgtcg
     2041 gcgacgaaca aattaagcgg ggactggggt ttattgaaac ctacggatgg gccgtggaca
     2101 acaaggatca aatttccccg ctaggctttg aagtcatttt tagtagcatg attaagtccg
     2161 cagaaaagtt ggacttgaat cttcccctga acttgcactt agtcaatcta gtcaaatgca
     2221 aacgggatag caccattaaa cggaatgtag aatatatggg agaaggcgta ggtgaattgt
     2281 gtgactggaa agagatgatt aagctgcacc aacgtcaaaa tgggagtctg tttgacagcc
     2341 ctgccactac tgccgccgcc ttgatctacc accagcacga tcaaaagtgt tatcaatact
     2401 tgaatagtat ctttcaacaa cataaaaact gggtgcccac tatgtacccc acaaaagttc
     2461 atagtttatt atgtttagtt gatacattac aaaatctggg ggtgcatcgt cattttaaaa
     2521 gtgaaattaa aaaagcgttg gacgaaatat accgtttatg gcaacaaaaa aatgaacaga
     2581 tttttagcaa tgtgacccat tgcgccatgg cttttcgact tctgagaatg agttattacg
     2641 atgttagttc agatgagctg gctgagtttg tagatgaaga acattttttt gcgactaacg
     2701 ggaaatacaa atcccacgtt gaaattttag aactccataa agcgtctcaa ttggcaattg
     2761 accatgaaaa agacgatatt ttggacaaaa taaacaactg gacgcgcgcg tttatggaac
     2821 aaaaactcct aaacaacggc tttattgacc gcatgagtaa aaaggaagtt gaattggcct
     2881 tacgcaaatt ttacaccact agtcacctcg cagaaaaccg tcgctatatc aaaagttatg
     2941 aagagaataa cttcaaaatt ttaaaagcgg cctaccggag tcccaacatt aataataaag
     3001 acttacttgc tttctccatc catgattttg aattatgcca agcccagcac cgggaagaat
     3061 tacagcaatt gaagagatgg tttgaagatt accggttgga tcaattaggc ttagccgaac
     3121 gttatattca cgcaagttat ctttttggag ttaccgtgat cccggagccc gagttatccg
     3181 atgcaagatt gatgtacgcg aaatacgtca tgcttctaac catcgttgat gatcactttg
     3241 aatcctttgc gtcgaaagat gaatgtttca acatcattga attagttgag cgttgggacg
     3301 attacgcttc cgttggctac aagtccgaaa aggtgaaagt gtttttcagt gttttttaca
     3361 aaagtattga agaactagcc accattgctg aaatcaaaca aggccggagt gtaaaaaatc
     3421 acctgattaa tctgtggttg gaattgatga aacttatgct tatggaacgc gttgaatggt
     3481 gtagcggaaa aacgattccg agtattgaag aatatctgta cgttacctct atcacttttt
     3541 gtgctaagtt gatcccccta tctacccaat actttctcgg tattaaaata agcaaagacc
     3601 tcttggagag tgatgaaatt tgtggtctct ggaactgctc cgggcgtgtg atgcgtattt
     3661 tgaacgattt gcaggatagc aaacgtgagc aaaaagaagt tagtattaat ctcgtaactt
     3721 tactaatgaa atccatgagc gaggaagaag ctatcatgaa aattaaagaa attttggaaa
     3781 tgaatcgccg tgagctatta aagatggttc ttgtccagaa gaaaggttcc caacttcccc
     3841 aattgtgcaa agatattttt tggcgcacaa gtaagtgggc ccatttcacc tactcgcaaa
     3901 ccgatgggta tcggatagcc gaagaaatga agaatcacat tgacgaagtg ttttataaac
     3961 cgttaaacca ctagagatct tcacattcta acgggagata ccagaacaat ggctagcctt
     4021 aatctacaaa cggaaaaact gtgttacgag gacaacgata acgatctgga tgaagaattg
     4081 atgcccaaac acatcgcttt gattatggat gggaatcgtc gctgggccaa agataaggga
     4141 ctagaagtct atgagggcca taagcatatt attccaaaat taaaagaaat ctgtgatatc
     4201 tcatctaagt taggcattca gattattacc gcctttgcat tcagtactga gaactggaaa
     4261 cgttccaaag aagaagtcga ctttttgctc cagatgtttg aagaaatcta tgatgagttc
     4321 agccgctctg gtgttagagt cagcattatt ggttgcaaaa gtgacttacc catgaccttg
     4381 caaaagtgta ttgctttaac ggaggagacc accaaaggga ataagggttt acacctagtg
     4441 atcgccctaa actacggggg ctattacgat atcctgcagg ccaccaaatc catcgtaaac
     4501 aaagccatga atggcctctt agatgtggaa gacattaata aaaacttgtt tgaccaggaa
     4561 ttagaatcca aatgtccgaa tccggacctg ttgattcgca cgggcggcga acagcgcgtc
     4621 tccaattttt tactgtggca actagcgtat actgaatttt attttaccaa tacattgttt
     4681 cctgacttcg gggaagaaga cttgaaagaa gcgattatga acttccagca gaggcatcga
     4741 cgttttggag ggcatactta ttaggcggcc gcgttgatcg gcacgtaaga ggttccaact
     4801 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     4861 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     4921 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     4981 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     5041 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     5101 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     5161 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     5221 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     5281 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     5341 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     5401 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     5461 tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     5521 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggggatc
     5581 ctctggttat tttaaaaacc aactttactc aggttccata cccgagaaaa tccagcttaa
     5641 agctgacata tctaggaaaa ttttcacatt ctaacgggag ataccagaac aatgaaaacc
     5701 cctttaactg aagccgtttc caccgctgac tctcaaggtc gctttctgag cagcaccgaa
     5761 ttgcaaattg ctttcggtcg tctacgtcaa gctaatgctg gtttgcaagc cgctaaagct
     5821 ctgaccgaca atgcccagag cttggtaaat ggtgctgccc aagccgttta taacaaattc
     5881 ccctacacca cccaaaccca aggcaacaac tttgctgcgg atcaacgggg taaagacaag
     5941 tgtgcccggg acatcggcta ctacctccgc atcgttacct actgcttagt tgctggtggt
     6001 accggtcctt tggatgagta cttgatcgcc ggtattgatg aaatcaaccg cacctttgac
     6061 ctctccccca gctggtatgt tgaagctctg aaatacatca aagctaacca cggcttgagt
     6121 ggcgatgccc gtgacgaagc taattcctac ctcgattacg ccatcaatgc tctgagctag
     6181 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     6241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6481 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6541 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6601 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6661 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6721 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6781 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6841 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6901 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6961 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7021 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7081 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7141 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8281 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8341 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgc
//
