LOCUS       sequence-137703-        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse Drp1 isoform b (minus residues 
            514-602) with C-terminal His-tag and PreScission protease cleavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Liu R, Chan DC
  TITLE     The mitochondrial fission receptor Mff selectively recruits 
            oligomerized Drp1.
  JOURNAL   Mol Biol Cell. 2015 Dec 1;26(24):4466-77. doi: 
            10.1091/mbc.E15-08-0591. Epub 2015 Oct 7.
  PUBMED    26446846
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-08-0591"; journalName: "Mol Biol Cell"; date: 
            "2015-12-1- 1"; volume: "26"; issue: "24"; pages: "4466-77"
COMMENT     Sequence Label: pET21-Drp1(deltaIB)
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgggcccc tggaacagaa cttccagcgg atccgcccaa agatgagtct
      121 ctcggatttc agcaataatt tgactggctc cttgtaatgc cttcagcata tccgctgctt
      181 cttttcttcg ttgggccatg tcctcggatt cagtcagaag gtcatctaat aaggatgact
      241 tatacagctg ccctaccagt tcactctgaa gagtatcttt cacatgatta accaaaaaat
      301 gcattactgc ctttgggaca ctgtcttgaa tattctttct gacaattaga aaatatgatt
      361 tgataagtct ttcaataacc tcacaatctc gctgttcctc tatattattg ttcattagcc
      421 cacaggcatc agcaaagtcg gggtgttttg tgttgatata cgctagctca attgccacta
      481 agttatgcac catttcattt gtcacgggca accttttacg aagaagacag gtcactactt
      541 caactatggc atcgtgaagt ttagggaacc gtaacaattc ctgtgtgctg taattgctac
      601 aatgctgaat gatcctctgc atctcctcat ggaccagctc cacacaccgt aggctgggct
      661 cttctagacg cttaatctga cgtttgacca gtaactcaaa tgaaacttca ggcacaaata
      721 aagcaggacg ggggccagta gcatttctga tggcagtcag gatgtcaata gtgttaaggc
      781 cacctagtgg gtccacagat tctaaggttc gcccgaaagt ctcatggaaa atataacaaa
      841 tcctagcacc accgcatagc tcagaagttt caatgtactt tgcggttcct tcaatcgtgt
      901 tacaatactc tgtggcaaat ttggtaataa gctggagtaa agtagcactt ttatcatcca
      961 ccggttcacc atagctattt agaagagact gatactgagc agctaagaca tttattcttg
     1021 ttttcagctc tggtaaacaa tctctgatat gatgcataag taacctattc agggtcctag
     1081 caagatactt tgttccattt ctgttggcca gagatgggta cttcttttga agaaa
//
