LOCUS       sequence-137705-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse Drp1 isoform b (699amino acids) with 
            C-terminal His-tag and PreScission protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Liu R, Chan DC
  TITLE     The mitochondrial fission receptor Mff selectively recruits 
            oligomerized Drp1.
  JOURNAL   Mol Biol Cell. 2015 Dec 1;26(24):4466-77. doi: 
            10.1091/mbc.E15-08-0591. Epub 2015 Oct 7.
  PUBMED    26446846
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-08-0591"; journalName: "Mol Biol Cell"; date: 
            "2015-12-1- 1"; volume: "26"; issue: "24"; pages: "4466-77"
COMMENT     Sequence Label: pET21-Drp1
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(74..97)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgggcccc tggaacagaa cttccagcgg atccgcccaa agatgagtct
      121 ctcggatttc agcaataatt tgactggctc cttgtaatgc cttcagcata tccgctgctt
      181 cttttcttcg ttgggccatg tcctcggatt cagtcagaag gtcatctaat aaggatgact
      241 tatacagctg ccctaccagt tcactctgaa gagtatcttt cacatgatta accaaaaaat
      301 gcattactgc ctttgggaca ctgtcttgaa tattctttct gacaattaga aaatatgatt
      361 tgataagtct ttcaataacc tcacaatctc gctgttctcg ggcagacagt tttcttgcaa
      421 ctggaactgg cacatctagc aaattgacag catggccttt ctgtggactt gctggcataa
      481 ttggaattgg ttttgatttt tcttcagcaa gtaattcttc agcttttgaa gttttcagca
      541 ttcctctcca gttgcctgtt gttggttcct gaccaccgtc tccaatccca ccacctgcag
      601 atgggacctt gtcccgtgat ccagctgaag gcagctctct tgctagcctg tttcttcttt
      661 gttcctctat attattgttc attagcccac aggcatcagc aaagtcgggg tgttttgtgt
      721 tgatatacgc tagctcaatt gccactaagt tatgcaccat ttcatttgtc acgggcaacc
      781 ttttacgaag aagacaggtc actacttcaa ctatggcatc gtgaagttta gggaaccgta
      841 acaattcctg tgtgctgtaa ttgctacaat gctgaatgat cctctgcatc tcctcatgga
      901 ccagctccac acaccgtagg ctgggctctt ctagacgctt aatctgacgt ttgaccagta
      961 actcaaatga aacttcaggc acaaataaag caggacgggg
//
