LOCUS       sequence-137708-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse Mff residues 1-61 with C-terminal 
            GST-tag and PreScission protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Liu R, Chan DC
  TITLE     The mitochondrial fission receptor Mff selectively recruits 
            oligomerized Drp1.
  JOURNAL   Mol Biol Cell. 2015 Dec 1;26(24):4466-77. doi: 
            10.1091/mbc.E15-08-0591. Epub 2015 Oct 7.
  PUBMED    26446846
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-08-0591"; journalName: "Mol Biol Cell"; date: 
            "2015-12-1- 1"; volume: "26"; issue: "24"; pages: "4466-77"
COMMENT     Sequence Label: pET28-Mff(1-61)-PP-GST
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(136..789)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(156..178)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(805..828)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgagtcaa tccgattttg gaggatggtc gccaccacca aacgtggctt gccagccctg
      181 caaaggccat gctatatact tgctggattt caagtactta tcaatttgtg ggatagcttc
      241 aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc aggcacattg ggtccatgta
      301 taaaacaaca tcaagagcgt catacaacat gaagtcagga tgggttacat gatcaccatt
      361 taaatatgtt ttatgacata aacgatcttc gaacattttc agcatttcag gtagcttgct
      421 aagaaaatca actttgagag tttcaaagtc tttactatat gcaattctcg aaacaccgta
      481 tctaatatcc aaaaccgctc cttcaagcat tgaaatctct gcacgctctt ttggacaacc
      541 acccaacatg ttgtgcttgt cagctatata acgtatgatg gccatagact gtgttaattt
      601 aacatcacca tcaatataat aaggaagatt gggaaactcc aaacccaatt caaacttttt
      661 gtttcgccat ttatcacctt catcgcgctc atacaaatgc tcttcatatt tttcttcaag
      721 atattccaaa agaagtcgag tgggttgcac aaggccctta attttccaat aacctagtat
      781 aggggacatt gcggccgcaa gcttgggccc ctggaacaga acttccagcg gatccgatcc
      841 tgtaaccacg atcctctctg ggacttgcat tatcacactg gcatttggaa ctccatcttg
      901 gaactcttgc tccaggtcag catt
//
