LOCUS       sequence-137728-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.25 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6145..6162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6188..6309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6225..6244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6279..6298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6347..6364)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6518..7106)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6598..6617)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7277..8068,8069..8137))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7900..7919
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8138..8242)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8310..8328
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8366..8388)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8390..8410)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8395..8725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8576..8711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8638..8657
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8862..8881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8948..9403)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9085..9106)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9297..9316
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9529..9551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9543..9560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9544..9560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgctactgcc aacttcacac
     1621 aacccgctca gcggaaacac ggcatacgaa atgaatatgc ccaacgaacg cctcaaatgg
     1681 ctaatgctgt tcgcagctgt tgccctcatt gcttgtggta gtcagaccct agctgccaat
     1741 cccccagatg ccgaccaaaa aggacccgtc ttcctcaagg aacccaccaa ccgcattgac
     1801 ttctccaact ccacgggcgc agagatcgag tgcaaggcca gcggcaatcc catgcccgag
     1861 attatttgga tcaggagcga cggtaccgcc gtgggtgatg tgcccggatt gcgtcagatc
     1921 tcatccgacg gcaagctggt cttccctccc ttccgcgccg aggactaccg ccaggaggtc
     1981 catgcccagg tgtacgcctg cctggcccgc aaccagttcg gatccattat ctcccgggac
     2041 gtccatgtgc gagccgttgt caatcagttc tatgaggcgg agattatgac cgagtatgtt
     2101 atcaggggta atgctgccgt tctgaagtgt tccatcccgt cgttcgtggc tgattttgtt
     2161 cgagttgagt cctggatcga tgatgagggc aatgtcttgt ccttttcgga caattacgat
     2221 ggaaagtatc tggtattgcc ctctggagag ctgcacatcc gtgaggttgg acccgaggac
     2281 ggatacaaga gctaccagtg ccgaaccaaa catcgtctga ccggagaaac ccgattaagt
     2341 gccacaaaag gacgattggt catcacagaa cctgtgggca gtgttgcgcc taagatcagc
     2401 gtagaaaatc gtctgaaaaa tgcagaggct cgaattggtt ccaccttcac cctcttttgc
     2461 cctggtcaag cctatccagt acccttcttt agatggtaca agttcatcga aggaacaacc
     2521 cgtaaacaag ctgtggtgct aaatgaccgt gtgaagcagg tctctggtac tctcattatt
     2581 aaggacgcag tagtcgagga ctccggaaaa tacctgtgcg tggtgaacaa ttccgtggga
     2641 ggcgaaagcg ttgagactgt gcttaccgtc actgcccccc tatccgctaa gatcgatcct
     2701 cccacacaga cagttgactt cggacgcccc gccgtattca cttgccagta cactggaaat
     2761 cccatcaaga ccgttagctg gatgaaggac ggcaaggcta tcggacacag cgaacccgtg
     2821 ctgcgcatcg aatccgtgaa gaaggaggac aagggcatgt accagtgctt cgtaagaaac
     2881 gaccaggaat cggcggaggc gagtgctgag ctgaagctcg gaggccgttt cgatcccccc
     2941 gtcatccgac aggccttcca ggaagagacc atggaacccg gaccaagtgt attcctcaag
     3001 tgcgtcgccg gcggaaaccc cacgcccgaa atttcctggg aactcgacgg caagaaaatc
     3061 gccaacaacg atcgctatca ggtcggacag tatgtgacgg tcaacggaga tgtggtttcc
     3121 tatctgaaca taacctcggt ccacgccaac gatggcggtc tctacaagtg catagccaag
     3181 agcaaagtgg gcgtggccga acactcggcc aagttgaatg tatatggact gccctacatc
     3241 cggcaaatgg agaagaaggc aattgttgct ggagaaaccc tgattgtcac atgtcctgta
     3301 gctggatacc ccatcgattc gattgtctgg gagcgagata accgtgccct gcccatcaac
     3361 cgcaaacaga aggtcttccc caacggaacg ctgattatcg agaatgtgga acggaactca
     3421 gaccaagcga cctacacttg cgttgccaag aatcaggaag gatactctgc tcgaggatct
     3481 ctggaagtgc aagtcatggt tccacccaaa attacgccct tcgacttcgg tgacgctccg
     3541 acgaatgtgg aggactctgt ttcggtcatg tgcctgattt ccaatgggga cttacccatc
     3601 gacatcgagt ggttctttaa cgactacgga attagttcgt actccggaat aaatgtagtt
     3661 aaaggtggca agaggaacag catgctaagt attgatagtg tccaggctcg tcatgccggg
     3721 aagtatagtt gtcgggctaa gaaccacgct gctgctgtaa attataccac cgaactaata
     3781 gttaatgtac ctcccagatg gatcctcgag cccaccgata aggccttcgc ccagggatcc
     3841 gatgccaagg ttgaatgcaa ggctgatggc ttccccaaac cccaagttac atggaagaaa
     3901 gcagttggcg acacccccgg agagtacaag gatctaaaga agagcgacaa catccgcgtg
     3961 gaagagggca ctctgcatgt cgacaacatc caaaagacca acgagggcta ctatctgtgc
     4021 gaggctatca atggaatagg ctccggcctc tcggctgtga ttatgatcag cgttcaggcc
     4081 cctccagagt tcacggagaa gctgcgcaac cagaccgccc gacgaggaga acccgccgta
     4141 ctccagtgcg aggcgaaggg cgagaagccc attggcatct tgtggaacat gaacaacatg
     4201 cgactggacc caaagaacga caatcggtac accattcgtg aggagatcct ttccaccgga
     4261 gttatgtcca gtctgtcgat caagcgcacc gagagatccg actccgccct attcacctgt
     4321 gtcgccacta atgcctttgg atccgatgac gccagcataa atatgattgt ccaggaagtt
     4381 ccggagatgc catatgcttt gaaggtactc gacaaatccg gacgttccgt gcagctgagc
     4441 tgggcgcaac cctacgatgg caactcccct ctggacaggt acatcattga gtttaagcgt
     4501 tctcgtgcct cctggagcga aattgatcgc gtcatcgtgc ccggacacac cacagaagcc
     4561 caagtgcaga agctcagccc cgccaccacc tacaacattc gcatcgtggc cgaaaacgcc
     4621 atcggcacat cccaatcctc tgaagcggtc actatcatca ctagtggatc ttccggaatc
     4681 atcctagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga ggccctgggt
     4741 gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat cttcctgggc
     4801 gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca gaagaaggac
     4861 aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc tctgtccaag
     4921 acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc ctacctgtgc
     4981 ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt taaccagtgc
     5041 aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa agcagggaag
     5101 tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg cacctacgcc
     5161 cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc ccgccaggag
     5221 gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt gatcctaggt
     5281 gggggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc agatgactac
     5341 agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct ggcgaagcgc
     5401 cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct ggacccgtct
     5461 gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat ccaccgagac
     5521 tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct gctgagcagg
     5581 aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa
     5641 agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat tgagagggcg
     5701 ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca ctcccacgtc
     5761 ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc ccctggcaag
     5821 gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg ctatgtgctc
     5881 aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga gtatcggcag
     5941 cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg
     6001 cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat agcgcacgtc
     6061 atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc ccccgccggc
     6121 accaccgacg ccgcgcaccc gggtcatcat caccatcacc attaatctag agatcttatt
     6181 aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6301 tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg ctcactgact
     6361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7321 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7381 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7441 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7501 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7561 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7621 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7681 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7741 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7801 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7861 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7921 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7981 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8041 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8101 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8161 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8221 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8281 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8341 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag gaatgtgtgt
     8401 cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca
     8461 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8521 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8641 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8701 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcgtccc
     8761 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     8821 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     8881 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8941 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     9001 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     9061 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     9121 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     9181 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     9241 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     9301 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     9361 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     9421 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9481 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9541 gttgtaaaac gacggccagt gaatt
//
