LOCUS       sequence-137735-        6242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Liu XK, Lin X, Gaffen SL
  TITLE     Crucial role for nuclear factor of activated T cells in T cell 
            receptor-mediated regulation of human interleukin-17.
  JOURNAL   J Biol Chem. 2004 Dec 10. 279(50):52762-71.
  PUBMED    15459204
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Dec 10. 279(50):52762-71."
COMMENT     Sequence Label: pGL3E-hIL-17prom(-1125)-Luc
FEATURES             Location/Qualifiers
     source          1..6242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1214..2866
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1280..1298)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2907..3028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2918..2937)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2972..2991
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3350..3370
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(3574..3591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3745..4333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3825..3844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4504..5295,5296..5364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5127..5146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5365..5469)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5496..5951
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5583..5602)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5793..5814
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6082..6130
                     /note="synthetic polyadenylation signal"
     misc_feature    6144..6235
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6184..6203
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt ggatctcagg acaaacaggt ccaggatccg gcaaactaat
       61 tttacactcc agccattgag ttggaactac tggccagcct cccccgagtt agcatgtaga
      121 atatgggata ccagctgagt gcctgagagt tatcattcac ctcagtgggg gtaggggcgg
      181 agaagggtga catatagcca gccacatcta tatccactgg cccttccttg tcctagtcct
      241 ctgtattcct gagaaggaac tattctcaag gacctgagtc caagttcatc ttacttagag
      301 tacagagaaa agaaccgcta actccttctc tctttccccc atcatgtctc ctctcctttc
      361 tagttctcat cactctctac tcccccctgc cccccttttc tccatcttca tcacctttgt
      421 ccagtctcta tccccatttt caattccttc ctcaaaacac caagttgctt ggtagcatgc
      481 agggttggaa catgccttta acagaaaatc tcgtgtctct tgaacctagt tatttattcc
      541 ttgagcagag tagatattca acaaaagaat tgttaaattc aattaaatag gatatatctt
      601 attattaaat atttttttca ttttttgttt acttatatga tgggaacttg agtagtttcc
      661 ggaattgtct ccacaacacc tggccaagga atctgtgagg aaaagaaaga tcaaatggaa
      721 aatcaaggta catgacacca gaagacctac atgttacttc aaactttttc ttcctcatga
      781 accattaaaa tagagcataa ctcttctggc agctgtacat atgttcataa atacatgata
      841 ttgacccata gcatagcagc tctgctcagc ttctaacaag taagaatgaa aagaggacat
      901 ggtctttagg aacatgaatt tctgcccttc ccattttcct tcagaaggag agattcttct
      961 atgacctcat tgggggcgga aattttaacc aaaatggtgt cacccctgaa cccactgcga
     1021 cacgccacgt aagtgaccac agaaggagaa aagccctata aaaagagaga cgatagcgct
     1081 acattttgtc catctcatag caggcacaaa ctcatccatc cccagttgat tggaagaaac
     1141 aacgatgact cctgggaaga cctcattggt ggtgagtcaa gcttggcatt ccggtactgt
     1201 tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     1261 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     1321 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
     1381 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     1441 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     1501 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     1561 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
     1621 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
     1681 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     1741 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
     1801 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
     1861 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     1921 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     1981 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
     2041 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     2101 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     2161 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     2221 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     2281 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     2341 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     2401 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     2461 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     2521 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     2581 catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga
     2641 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     2701 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     2761 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     2821 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg
     2881 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2941 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3001 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     3061 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     3121 aatcgataag gatctgaacg atggagcgga gaatgggcgg aactgggcgg agttaggggc
     3181 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     3241 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     3301 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     3361 cacacattcc acagcggatc cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     3421 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     3481 tgcaactcgt aggacaggtg ccggcagcgc tttatgactg tcttctttat catgcaactc
     3541 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4381 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4441 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4501 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4561 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4621 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4681 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4741 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4801 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4861 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4921 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4981 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5041 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5101 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5161 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5221 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5281 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5341 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5401 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5461 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     5521 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5581 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5641 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5701 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5761 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5821 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5881 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5941 gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6001 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg
     6061 ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct gtgtgttggt
     6121 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
     6181 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctctatcga
     6241 ta
//
