LOCUS       sequence-137744-       11587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acetobacter aceti 1023 gDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Kappock
  TITLE     Additional Acetobacter aceti 1023 ORFs associated with functional 
            complementation of an alanine racemase (alr-, dadX-) auxotroph
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK215
FEATURES             Location/Qualifiers
     source          1..11587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(12..45)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        52..70
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9570..9589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9571..9589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        9627..9731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9732..9800,9801..10592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10763..11351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11252..11271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11505..11522
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccggtacc cataacttcg tatagcatac attatacgaa gttatgcggc caattaaccc
       61 tcactaaagg gagcggccgc ggatcattaa accgaatgtt cagccgggct tcggcccgtg
      121 cgggaataac attggtcgcc ccattgccta catccacact ggtaacctga aggctggagg
      181 gttcaaaata ctcggtaccg ttatccagcg gtgtggcgcg caacgcttcc agcaccgcca
      241 gcaggcgatg caccgggtta tctgcccggt ggggataggc cacatggccc tgcgtacctt
      301 ccaccacaat atgcgcgttc aagctgccac ggcggccaac cttcaccatc tcgcccaaca
      361 ccttggggtt ggtgggttca cctaccagac agtaatccgg gatctgattg tgctgcgcca
      421 tccattccag cacctttacc gtgccatagg tggcggggcc ttcttcatcc ccggtgatca
      481 gaaaactgat ggagcctttg cccggcccag ccgccacatg ccgcgctacg gcagatacaa
      541 aagccgcaat accgcccttc atgtcacacg cgccacggcc atacagcacg ccatctacaa
      601 tatccgccgc ataagggggg tgagaccaat cggcctcgtt ccccggcggc accacatcgg
      661 tatggcccgc gtagcagata tggggactgc ccgtgcccag acgagcaaag aaattgggcg
      721 tacgctccgc accctcacca aacggaagat gcgtgacctc aaaccccaga cgctccagca
      781 tggctgcaag cagatgctgt gatgcgccgg gatctggcga gacagaaggc agacggataa
      841 gcgcctgcgc cactccaacc ggatctgtca gtgccagatt ggccggaata ttcacctgcg
      901 ctgcgtccgc catgcttttc tgcctctttt ttgcttttag tctgcctgat cagtcacgca
      961 gcagatcgtt aatggaagtt ttggaacgtg tgcgttcgtc cacccgcttc acaattacgg
     1021 cgcaagccag agcaggattg gggcgaccat ctgggccaac cggcttggaa gacggcatgg
     1081 tgcccggcac aacaacggaa taagccggaa cgcggcccat ataaatttca cccgttgcac
     1141 ggtccacaat cttggtggaa gcgccaagga acacacccat ggacagcacg gagccacgtt
     1201 ccacaatcac gccttctgcc acttcggaac gtgcgccgat gaagcagttg tcttcaataa
     1261 tcaccggggc agcctgcagc ggttccagaa caccaccaat gcctgcgcca ccgctgatat
     1321 ggcagttctt gccaatctgg gcacagctgc caacagtggc ccatgtgtcc accatggtgc
     1381 cgctatctac gcgggcgccc acgtttacaa agctgggcat cagcacagca ccgggggcaa
     1441 taaaggcgga gcggcgcaca acagcgcccg gcaccacgcg gaagccagcc ttatcaaaac
     1501 gggcctgatc ccagccagca aacttcagcg gcactttgtc ataagccggg gcaccggcgg
     1561 cgccaccggg aatggcaacg ctatcgttca gacggaagga aagcagaaca gcttttttaa
     1621 gccattcatt cacctgccag ccatcttcct tgggttcggc cacacgcagc gcgccagaat
     1681 caagggcttc cagagccgtt tcaatggctt tgcggtcttc ccccgttgta gcggaggaaa
     1741 tctggtcccg cttttcccac agggcttcaa tatgcgcgcg caaggtttcg ttggtcataa
     1801 cacaccgtca gacaggatgg acaaacaccg ggcctgcacc acagccccgg cagaaaaata
     1861 cagagcgacc atgcggacac acacgcggcg tgtcaacgca tggcaccccg gaaaatggca
     1921 ggcaaccgcg gcgttattcc gcccggatca tggtgccgga gccagatgcc gtaaacagtt
     1981 ccagcaagca ggcatggggc atacggccat caataatcac ggctgcacgc gcaccggcct
     2041 gcacggcctg aatgcaggtt tccaccttgg ggatcatacc gccggtaatt acaccatcct
     2101 caatagcctg catggcctgc gcggctgtga gttcaggtat cagcttgcca tcggcatcca
     2161 gcacaccggg cacgtctgtc agcatcagca ggcgtgtggc atgcaccgca ccggctacag
     2221 caccggcagc cgtatcagca ttgatgttgt aggtggcgcc atcttcccca aagcctactg
     2281 gtgcaataac cggaataagg ccggaaccgg agagcgcata aatcacgcgc gggtccacct
     2341 ttacgggttc gcccacaaag cccagatcca gcacgcgctc aatctggctt tccgtatccc
     2401 gcaccgtgcg ttgcagcttg cgggcggtaa tcattttacc gtccttgccg gaaataccaa
     2461 cggccagcgc accttcacgg ctgataagct ccgccacttc cttgttcacg gaaccagcca
     2521 gaaccatctc gataacatct accatattgg cgtctgttac acgcaggcca tccacaaagg
     2581 tggagggaat ttccagccgc ttgagcatct ggttaatctg cgggccgccg ccatgcacca
     2641 caaccgggtt gatacccacc agcttgagca gcgcaatatc ccgcccaaag gttttggcca
     2701 gatctgcatt gcccatggcg tggccaccat acttgaccac aatggtatcc cccgcatagc
     2761 ggcgcagata cggcagggct ccggccagaa tggcggcttc ctgcgccgca tcatgatggg
     2821 aatgggaaaa aggaacccgt ggttctgtca tgctctgcac ttcctgcttg tcatcaatca
     2881 gatcatggcg ccttgggcag ggcaaattct gccagttccg cccgcaagcc ctcaatacct
     2941 tcacctgtct ggctgcttgt taaggacagg tagggaaaag ccgcagggtg gcggcgaatt
     3001 tccgcttcaa cttctgcctg cttgcgtgcc agcgggccgg gtttaacgct atcgcatttg
     3061 gtcagcacca cctgaaagct gacggctgcc ttatccagca ggtccatcac ttccagatca
     3121 tgcgtcttca tttccacccg cgcatcaagc agcagcacaa cacgctgcaa gcttggccga
     3181 ccgcgcaggt aatcaaacat catatcctgc cagtcttcct ttacggattt ggcagccttt
     3241 gcgtagccat aacccggcat atccaccagc atcagccggt ctgccagatt gaagaagtta
     3301 agctgcttgg tgcgccccgg ctcggaagag gcccgcgcca gcgttttgcg acccgtaagc
     3361 gcgttcacca agctggactt gcccacattg gagcgcccgg caaacgcaat ttccggcagg
     3421 gtttgcggcg gcagttgccc cagcttttgc gcgccaaaca caaattcgca tgaacccgca
     3481 aaaagccgcc gcccctcttc cagggcggcg gcaatacggg cttcatcctt tagttctcgg
     3541 aaagaggatg cggcatcatc tgtcatttgg gcttaaccag ttggggtttg gcatctgccg
     3601 ctttcaggcg ggagataatg ataacctgct gcacagccgt aaggatgttg ctccagcagt
     3661 aataaatcac cagcccgacc ggctgccgcg ccatgaagaa tgtgaacagc agcggaatga
     3721 acatcatcac cttttgctgt gatgggtcca tgttggcaga gctggagctg accttctgca
     3781 tcaggaacag cgtggcccca tacatgatcg gccaagcgcc cagcgccaga aacggcgtga
     3841 tgtgcgtggg atcaaacgga ataagaccaa acaggttgaa caggttggtg gggtctggcg
     3901 cagaaagatc cttcacccag cccacaaacg gcgcgtgccg catttcgatg gtgacataca
     3961 gatccttata caagcaccag aacacaggcg cctgaataag cagcggcaga aagcccccga
     4021 acagattaac gttttcctgc cgatacagcg ccataatctg ctgctgcatg gccatcgggt
     4081 catccttcag gcgggagcgg atcaggtcca cctgcggctt caggcgcgct gttttccatg
     4141 tcatacgcat ctgcttgatg gtgagcggca ggaaggccac cttcacaatc acggtgaacg
     4201 taagcagagc cagaccaaag ctgcccagat gttcgttcag ccagtccagc acaaaaaaca
     4261 cgggacgggt aaggaaggaa agccagccaa agtccactgc tttccagaac atgggcacgt
     4321 gcaggtcttt ttcatactgc tcaagcagcc gcacttcctt ggcgcccgca aacacgtggg
     4381 cctcggtaga ggctgtctgc cccgcagcca cctgcacagg tgccgtagcc gtaaagccaa
     4441 cctgatacgt gccttgggcc gcctgaaaac cataggtgcc aatcacgttg gcgttctggt
     4501 ctggcagcac ggccatcagc cagtatttgt ctgtaatacc agcccaaccg ccggtgccct
     4561 gatggctcca tgcaatgttg tctggcggta cgccaccggt gcgcaccgtt ttgtaggaac
     4621 cttcgttcag gcggccatca atcaccgaga tcgggccttc atgcaccagc atgccaccgg
     4681 tttcttccgg cgtgtagcca cggttcacac ggtagaaggg atacagcgcc acagcctgcc
     4741 cggtgctgtt atgcacctgc tggctgatgg tgaacatgta gttctgatcc accgacaggc
     4801 ggatatcaaa gatcagcccc tgtccgttat cccatgaaag ggtgagcggg tgttcggcat
     4861 ccatttgggt gccagaggct ttccacaacg tgttggcatc tggcagacgc acggacacat
     4921 cggacggtgc acgccagcca aaatccacat aatctgcatg ggtggacgca gaattaagaa
     4981 cacgcacatc cgggctgtct ggctttacgg tttcccgata atgcttgagc accagatcat
     5041 ccagccgtgc gccacgcagg tttagcgtgc cctgcacggc aggggcgtta atggccacac
     5101 gggtttcggg gccatcctgc acggggggcg tggcttgggc ctgcgcgttt tcagccgggg
     5161 cagcagcatc agaagatgca ggcggcgcta cggcatgcac gcccggctgc ggcggtggca
     5221 caggctggtg ctgcgggaaa aagtaatcat acccgataag taccagagct gacatcaatg
     5281 ttgccagtat cagacgtttt gaatccataa gctccggcct tgcggccccc tgcacatatc
     5341 aaattggaaa acttaacacc cgtaaaaaac ccggagtctt aaacggcata caggttccag
     5401 cgcaggacgc acacgcatgc aaggcaggat attcaggaat cccgctggca caacgccaat
     5461 gctttacgga aatcagccac caacgccgca aatgggcgct ttatggtggc gctacggcct
     5521 ataagaacaa ggtccagacc gtttagcggc gtatcggctt ctacaatacg cacaacttcg
     5581 cgcaacctgc ggcgcacgcg gttacgcacc acggcattgc caaccttttt ggtaaccgta
     5641 aagcccacac gcgcgacatc cgtatccgga cgccggaaaa gctgcaaaac aagccccgac
     5701 acaggcgctt tacgcccgcg agaagccaga aagaggaatt gcttcctctt tttaagccgc
     5761 tgagatctgg cagactctgc ctctggctgc tcctgcgctg ctggcccgtc catcaagccg
     5821 gactgccggg cagttcttac gcggaaaggc gcttacggcc cttggaacga cggttagcaa
     5881 tgatcttgcg gccaccaacg gttgccatac gcgcgcggaa cccgtgacga cgcttgcgaa
     5941 ccagccggga cggttgataa gtgcgcttca tggcggcact cccttactat ttgaatacag
     6001 ttatccggtg gcctgtaaaa gaccacgcct cttgaagctg cggcttatag agttcagata
     6061 aggaaccgtc aatctatacg atagagtcgt taccgcaacc ttggggcaga atctgccaca
     6121 agtgggagaa aaacatgaac gccatgctct ttcctgtccg tgccgcagcc gttattttct
     6181 gcctgcctgt ggcgggcgct ctgctggccg ggctgctggg gctgtccttc cccgcagcat
     6241 tggggcctgt tgtatgtgcg gcaggcttgg gcgcatggct gggcataaaa cttcagccac
     6301 acgcggcccc tcctgccccg gttgcagcac ctcagccccc agctccagca gtagattcca
     6361 aactgcggca tgatattcgc ggcattgttt cccccgccat gctggcagcg gaacagcttg
     6421 atgcatattc cgacccagcc gtgcagaaag ccgccaaaac catcaatgat tcactagacc
     6481 gccttacagc gcggctgaag cagcgcccgc aaggctgaac agcgctttat tcagcgcgtt
     6541 caatcagctc aaccttgtag ccatcggggt cttctacaaa ggcaatcacg gtggtgccaa
     6601 attttaccgg cccagcttcc cgcgttacct tgccaccacc ggcccgcaca cgttcaacca
     6661 tttccgccac attgggcaca cccacggcaa aatggccaaa gcctgtgccc agctcgtagc
     6721 catcatcctt gccccagtta taggtcagct caatttctgc ctgtccggcc gcgttatctg
     6781 catagcctat aaacaccagc gtataacgcc cttccggcac atccttccgg cggagttcgt
     6841 gcatgcccag cagtttgtaa aacgccagac ttttttccag attacgcacc cgcaccatgg
     6901 tatgcagaaa acttgccatc tgtttgctcc ttatcttaaa ggctcaggca gccctatgcc
     6961 ctgtgtggca taatcatccg ggttaaaggc caaaagaaac acgcccaacc tgtttgcagc
     7021 ctctatgcac ccttgcgtat ctgtcagcag cgtatgggcg gcctcaaacg caatgccacg
     7081 taagcctgct gcatgggcgt tctgcactgt tacaggccct attgtgggca tatccgcgcg
     7141 cttttcctgc cctggcttca gcattttaac cagaacaccg ccttctcccg gctgcctgca
     7201 ttctccggcg cgggccagca tacggtctgt gccttccatg gcttccactg ccagcaccac
     7261 gccattctgt accacacagc cctgcccgat atccaaccgc cccagcgcct gcaccacggt
     7321 tatgccgcgc tgaatatcgg cctgcgcctg tgcatctggc tgcacacgcc ccaacacacc
     7381 gggctgcgcc acagcttgtg tgagaaactc atgcgcccca cgaatacgaa agccttcttc
     7441 ccccagaatc cgcacaaggg ccgccagcag gccatcatcc cccgcaaaca gggcgcggcc
     7501 caaacgggcc agaatacgcg cgccctcggc atccgggcac agattacgca aggagggtcg
     7561 acggataggc ccgataagca ccagatccgt gcagttatgt tgccgcaaaa gggagagcat
     7621 acgcccggca gcacccagcc ggacacattc gtgcggccaa gggccaatca cttcgggttc
     7681 ggcaaaacct tcaaacccaa gaataaaaac aggccgccct gttgcggcgg ccgcttctgc
     7741 caccctaccc ggcagagggc caccacctgc aagaatgcca acggcagagg ctgtgctcac
     7801 gcgccctcgg tttctgcgcg ttccagatcc tgccgtgccg ggcgcacaag accacgccgg
     7861 ctgggggcat ctataaactc cagaatctca cgcacacggg ggttatcacc aaattccgca
     7921 cgcacacgtt ccagccgcac agaaaacgga tcttccgtgc acccattctt ggggtagagt
     7981 gtcaggaacg ccttgcgcat gcggtgaatt tcatcagact gcacgccatt gcggcgcagc
     8041 cagatccaat gcaggccaat cagccgcgca cggttgccca gcacactacc ataagggata
     8101 acatcggctt ccaccccagc cacaccgccc acaagagccg catgcccgat gcggacaaac
     8161 tggtggatgg cggcagaacc catgatacgg gcatcatcct caatggtcac atgcccgccc
     8221 atcaccacgt tatttacgat aataacgcgg tcacccagca cgcaatcatg cgctacatgg
     8281 gcgttggcca taatcagcac attctggccc acccttgtca agccagaacc cgtggccgtg
     8341 ccacgatgga tggtaacgtg ctcacgcacc accgtgcccg ccccgatctc gcaccgggtt
     8401 ggctcacctt tgtacttcag atcctgcgga gccatgccca cggtgcaaaa cggaaaatag
     8461 cgcgaccctg cccccagacg tgtgtggcca tcaaccacca cgtgagagat cagctccact
     8521 ccatcttcaa tcgtgacatc aggcccgaca gaacaccaag ggccaataac caccccctgc
     8581 ccaagccgcg caccgggagc aacaagagcc gttgggtgga caacggttga tctggcttcc
     8641 tctgcagcgt gcaccaattc agttccttat ttccgttcac cgttggccgg gcagatttac
     8701 cctaccttac cctttgcaaa acaatctggg cactttcggg gcaaacctga cttgccttaa
     8761 cagaatgatg tgaacaggcc gggttctcaa cccataatca tggcgctgaa tgtggcttcc
     8821 gccacgacag tgccttccac acgggcttca ccgcggaatt tccacacatt ggagcggtgg
     8881 cgttccttgg caacatgaat acgcacctga tcccccggtt ctaccggccg gcggaacttg
     8941 gcgccttcaa tggtcatgaa atagaccagc ttgccctcaa aatccttgcc cagtgtcagc
     9001 acaaccagcg ttgcggcggt ttgcgccatg gcttccacca gcaacacgcc cggcataaca
     9061 gggcgggccg gaaaatggcc ctgaaagaat ggttcattga ccgaaacatt cttgatacct
     9121 gttgcctctt ctcccaggac gatatcttcc atccggtcga tcagaagaaa aggatatctg
     9181 tgcggaatcg cctgcatgat acggttgacg tcaattctgt cgatcgtctg agcgtcctgc
     9241 tgcgattctt gtttcgtctc cacgtccccg aaaccaccct ttgcgccggt gtctggcacc
     9301 gaccttagtt taacccttgt ccgaaccgtt tttggccgat ccagacgctt tttttgccag
     9361 cttgcgcaat accgccacgt tccggaaaaa ttccctgaac ggcatagccg ggctgccaat
     9421 cacatccgcg ccgccttcta tatccgacat cacgccacat tgcgcgccaa tacgcgcctt
     9481 tgcaccaatg cggatatgcc cgataaggcc agcctgcgcg gcaattgtta catagtcttc
     9541 cagcacggtg gagccaggat ccgcggccgc cctatagtga gtcgtattag gccgcgacgt
     9601 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtcata
     9961 cactattatc ccgtattgac gccgggcaag agcaactcgg tcgccgggcg cggtattctc
    10021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10261 acaccacgat gcctgtagca atgccaacaa cgttgcgcaa actattaact ggcgaactac
    10321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10561 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    10921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11341 gcctatcgaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11521 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11581 aatgcag
//
