LOCUS       sequence-137745-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); 
            N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe 
            mutant (AaPurK-V319F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK552
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     97..116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        97..115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    116..140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
       61 gacgctctcc cttatgcgac tcctgcatta ggaaattaat acgactcact ataggggaat
      121 tgtgagcgga taacaattcc cctgtagaaa taattttgtt taactttaat aaggagatat
      181 accatggtga gcgaaaccgc ccccctgccc tctgcttcca gtgctttaga agataaagca
      241 gcctctgccc ccgttgtggg cattatcatg ggcagccagt cagactggga aaccatgcgc
      301 catgcagacg cattgctgac agagctggaa attccgcatg aaaccctcat tgtttcagca
      361 catcgtacgc cagaccggtt ggcggattat gcgcgtacgg cagccgaacg cggcctgaat
      421 gtgattattg ctggggccgg tggagctgca catctgccgg gtatgtgtgc ggcatggaca
      481 cgcctgcccg tgctgggtgt tccagtagaa tctcgcgctc ttaaaggcat ggacagcctg
      541 ctttccattg tgcagatgcc cggcggcgtg cctgtgggca ctttggctat tggggcatcc
      601 ggcgccaa
//
