LOCUS       sequence-137751-        3153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides 6xUAS (upstream activating sequence) as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: p6xUAS (GB0179)
FEATURES             Location/Qualifiers
     source          1..3153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(204..222)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(205..222)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(240..256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(240..256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(253..275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    264..280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(288..294,295..312,313..318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    333..354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(471..488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(642..1230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(722..741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1401..2192,2193..2261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2024..2043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2262..2366)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2444..2899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2581..2602)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2793..2812
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(2876..3153,1..244))
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDWAMSG
                     LGDGGQYSARRTVLRSEDSTPLGGQYSARRTVLRSRDHSSPIEFPRPPWRPGACDVGPN
                     SPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN
                     GEWTRPVAAH"
     primer_bind     3025..3047
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3039..3056
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3040..3056
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3063..3082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3063..3081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3112..3121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgggagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
       61 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gtccccgaga ccactcatcg
      121 cccaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg
      181 cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
      241 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      361 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      421 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1381 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1441 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1501 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1561 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1621 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1681 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1741 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1801 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1861 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1921 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1981 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2041 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2101 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2161 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     2221 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2281 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2341 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg
     2401 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     2461 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2521 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2581 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2641 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2701 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2761 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2821 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2881 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     2941 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3001 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3061 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctccc ggccgccatg
     3121 gcggccgcgg gaattcgatg ggcgatgagt ggt
//
