LOCUS       sequence-137753-        9515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses humanized PdCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, 
            Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin 
            EV, Zhang F
  TITLE     Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas 
            System.
  JOURNAL   Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038.
  PUBMED    26422227
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2015 
            Sep 23. pii: S0092-8674(15)01200-3. doi: 
            10.1016/j.cell.2015.09.038."
COMMENT     Sequence Label: pY018 (pcDNA3.1-hPdCpf1)
FEATURES             Location/Qualifiers
     source          1..9515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        396..599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     550..570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4679..4726
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4733..4813
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4887..4904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4893..5117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5163..5591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5250..5269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5460..5481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5600..5620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5605..5934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5785..5920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5847..5866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6001..6795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6055..6074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6665..6684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6969..7090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7006..7025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7060..7079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7139..7155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7139..7155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7152..7174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7163..7179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7187..7193,7194..7211,7212..7217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7232..7253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7370..7387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7541..8129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7621..7640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8300..9091,9092..9160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8923..8942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9161..9265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9340..9359)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
       61 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      121 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      181 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      241 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      301 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      361 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      421 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      481 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      541 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      601 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      661 gggagaccca agctggctag cgtttaaact taagcttggt accgccacca tggagaacta
      721 tcaggagttc accaacctgt ttcagctgaa taagacactg agattcgagc tgaagcccat
      781 cggcaagacc tgcgagctgc tggaggaggg caagatcttc gccagcggct cctttctgga
      841 gaaggacaag gtgagggccg ataacgtgag ctacgtgaag aaggagatcg acaagaagca
      901 caagatcttt atcgaggaga cactgagctc cttctctatc agcaacgatc tgctgaagca
      961 gtactttgac tgctataatg agctgaaggc cttcaagaag gactgtaaga gcgatgagga
     1021 ggaggtgaag aaaaccgccc tgcgcaacaa gtgtacctcc atccagaggg ccatgcgcga
     1081 ggccatctct caggcctttc tgaagagccc ccagaagaag ctgctggcca tcaagaacct
     1141 gatcgagaac gtgttcaagg ccgacgagaa tgtgcagcac ttctccgagt ttaccagcta
     1201 tttctccggc tttgagacaa acagagagaa tttctactct gacgaggaga agtccacatc
     1261 tatcgcctat aggctggtgc acgataacct gcctatcttc atcaagaaca tctacatctt
     1321 cgagaagctg aaggagcagt tcgacgccaa gaccctgagc gagatcttcg agaactacaa
     1381 gctgtatgtg gccggctcta gcctggatga ggtgttctcc ctggagtact ttaacaatac
     1441 cctgacacag aagggcatcg acaactataa tgccgtgatc ggcaagatcg tgaaggagga
     1501 taagcaggag atccagggcc tgaacgagca catcaacctg tataatcaga agcacaagga
     1561 ccggagactg cccttcttta tctccctgaa gaagcagatc ctgtccgatc gggaggccct
     1621 gtcttggctg cctgacatgt tcaagaatga ttctgaagtg atcaaggccc tgaagggctt
     1681 ctacatcgag gacggctttg agaacaatgt gctgacacct ctggccaccc tgctgtcctc
     1741 tctggataag tacaacctga atggcatctt tatccgcaac aatgaggccc tgagctccct
     1801 gtcccagaac gtgtatcgga atttttctat cgacgaggcc atcgatgcca acgccgagct
     1861 gcagaccttc aacaattacg agctgatcgc caatgccctg cgcgccaaga tcaagaagga
     1921 gacaaagcag ggccggaagt ctttcgagaa gtacgaggag tatatcgata agaaggtgaa
     1981 ggccatcgac agcctgtcca tccaggagat caacgagctg gtggagaatt acgtgagcga
     2041 gtttaactct aatagcggca acatgccaag aaaggtggag gactacttca gcctgatgag
     2101 gaagggcgac ttcggctcca acgatctgat cgaaaatatc aagaccaagc tgagcgccgc
     2161 agagaagctg ctgggcacaa agtaccagga gacagccaag gacatcttca agaaggatga
     2221 gaactccaag ctgatcaagg agctgctgga cgccaccaag cagttccagc actttatcaa
     2281 gccactgctg ggcacaggcg aggaggcaga tcgggacctg gtgttctacg gcgattttct
     2341 gcccctgtat gagaagtttg aggagctgac cctgctgtat aacaaggtgc ggaatagact
     2401 gacacagaag ccctattcca aggacaagat ccgcctgtgc ttcaacaagc ctaagctgat
     2461 gacaggctgg gtggattcca agaccgagaa gtctgacaac ggcacacagt acggcggcta
     2521 tctgtttcgg aagaagaatg agatcggcga gtacgattat tttctgggca tctctagcaa
     2581 ggcccagctg ttcagaaaga acgaggccgt gatcggcgac tacgagaggc tggattacta
     2641 tcagccaaag gccaatacca tctacggctc tgcctatgag ggcgagaaca gctacaagga
     2701 ggacaagaag cggctgaaca aagtgatcat cgcctatatc gagcagatca agcagacaaa
     2761 catcaagaag tctatcatcg agtccatctc taagtatcct aatatcagcg acgatgacaa
     2821 ggtgacccca tcctctctgc tggagaagat caagaaggtg tctatcgaca gctacaacgg
     2881 catcctgtcc ttcaagtctt ttcagagcgt gaacaaggaa gtgatcgata acctgctgaa
     2941 aaccatcagc cccctgaaga acaaggccga gtttctggac ctgatcaata aggattatca
     3001 gatcttcacc gaggtgcagg ccgtgatcga cgagatctgc aagcagaaaa ccttcatcta
     3061 ctttccaatc tccaacgtgg agctggagaa ggagatgggc gataaggaca agcccctgtg
     3121 cctgttccag atcagcaata aggatctgtc cttcgccaag acctttagcg ccaacctgcg
     3181 gaagaagaga ggcgccgaga atctgcacac aatgctgttt aaggccctga tggagggcaa
     3241 ccaggataat ctggacctgg gctctggcgc catcttctac agagccaaga gcctggacgg
     3301 caacaagccc acacaccctg ccaatgaggc catcaagtgt aggaacgtgg ccaataagga
     3361 taaggtgtcc ctgttcacct acgacatcta taagaacagg cgctacatgg agaataagtt
     3421 cctgtttcac ctgagcatcg tgcagaacta taaggccgcc aatgactccg cccagctgaa
     3481 cagctccgcc accgagtata tcagaaaggc cgatgacctg cacatcatcg gcatcgatag
     3541 gggcgagcgc aatctgctgt actattccgt gatcgatatg aagggcaaca tcgtggagca
     3601 ggactctctg aatatcatca ggaacaatga cctggagaca gattaccacg acctgctgga
     3661 taagagggag aaggagcgca aggccaaccg gcagaattgg gaggccgtgg agggcatcaa
     3721 ggacctgaag aagggctacc tgagccaggc cgtgcaccag atcgcccagc tgatgctgaa
     3781 gtataacgcc atcatcgccc tggaggatct gggccagatg tttgtgaccc gcggccagaa
     3841 gatcgagaag gccgtgtacc agcagttcga gaagagcctg gtggataagc tgtcctacct
     3901 ggtggacaag aagcggcctt ataatgagct gggcggcatc ctgaaggcct accagctggc
     3961 ctctagcatc accaagaaca attctgacaa gcagaacggc ttcctgtttt atgtgccagc
     4021 ctggaataca agcaagatcg atcccgtgac cggctttaca gacctgctgc ggcccaaggc
     4081 catgaccatc aaggaggccc aggacttctt tggcgccttc gataacatct cttacaatga
     4141 caagggctat ttcgagtttg agacaaacta cgacaagttt aagatcagaa tgaagagcgc
     4201 ccagaccagg tggacaatct gcaccttcgg caatcggatc aagagaaaga aggataagaa
     4261 ctactggaat tatgaggagg tggagctgac cgaggagttc aagaagctgt ttaaggacag
     4321 caacatcgat tacgagaact gtaatctgaa ggaggagatc cagaacaagg acaatcgcaa
     4381 gttctttgat gacctgatca agctgctgca gctgacactg cagatgcgga actccgatga
     4441 caagggcaat gattatatca tctctcctgt ggccaacgcc gagggccagt tctttgactc
     4501 ccgcaatggc gataagaagc tgccactgga tgcagacgca aacggagcct acaatatcgc
     4561 ccgcaagggc ctgtggaaca tccggcagat caagcagacc aagaacgaca agaagctgaa
     4621 tctgagcatc tcctctacag agtggctgga tttcgtgcgg gagaagcctt acctgaagaa
     4681 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat cctacccata
     4741 cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc catatgatgt
     4801 ccccgactat gcctaagaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     4861 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4921 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4981 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5041 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5101 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5161 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5581 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5701 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5761 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5941 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6001 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6061 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6181 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6781 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6841 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6961 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7081 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7981 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9301 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9361 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9421 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     9481 ggttaggcgt tttgcgctgc ttcgcgatgt acggg
//
